171728

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 2102743..2103542 Replicon chromosome
Accession NZ_CP060953
Organism Escherichia coli strain EC8

Toxin (Protein)


Gene name yhaV Uniprot ID A0A0A6SPA6
Locus tag H9195_RS10230 Protein ID WP_000347275.1
Coordinates 2103078..2103542 (+) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag H9195_RS10225 Protein ID WP_001307405.1
Coordinates 2102743..2103078 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H9195_RS10210 (2098528) 2098528..2099298 - 771 WP_001058209.1 2-dehydro-3-deoxyglucarate aldolase -
H9195_RS10215 (2099314) 2099314..2100648 - 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
H9195_RS10220 (2101023) 2101023..2102594 + 1572 WP_001273752.1 galactarate dehydratase -
H9195_RS10225 (2102743) 2102743..2103078 + 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
H9195_RS10230 (2103078) 2103078..2103542 + 465 WP_000347275.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
H9195_RS10235 (2103597) 2103597..2104406 - 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
H9195_RS10240 (2104655) 2104655..2105935 + 1281 WP_000681933.1 tagatose-bisphosphate aldolase subunit KbaZ -
H9195_RS10245 (2105958) 2105958..2106431 + 474 WP_001336162.1 PTS N-acetylgalactosamine transporter subunit IIB -
H9195_RS10250 (2106442) 2106442..2107221 + 780 WP_000406209.1 PTS N-acetylgalactosamine transporter subunit IIC -
H9195_RS10255 (2107211) 2107211..2108089 + 879 WP_001295548.1 PTS N-acetylgalactosamine transporter subunit IID -
H9195_RS10260 (2108107) 2108107..2108541 + 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 2093595..2103542 9947


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17820.29 Da        Isoelectric Point: 9.8492

>T171728 WP_000347275.1 NZ_CP060953:2103078-2103542 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKVPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

>T171728 NZ_CP078069:c457633-457337 [Comamonas sp. NLF-1-9]
ATGTACACGATTCAGCGCACGGAACAGTTTTCTGGCTGGCTGGATGGCTTGAAAGACCGGGTGGCGCGAGGTCGCCTTGT
TCGTCGACTGGAGAAGGCGCAGCGCGGGCTGCTGGGTGACGTTCAGCATGTAGGCGAAGGCGTGTTTGAGATGCGCGAGT
TCTTTGGCCCTGGCTGGCGCATGTACTACGTTCAGCGCGGCGCGGTGTTGATTGTGATGCTGGGCGGGGGCGACAAGTCC
ACCCAGCAGGCTGACATTGCTGCCGCGCAGACTTTGGCTGCAACCCTTGAGGAGTGA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT171728 WP_001307405.1 NZ_CP060953:2102743-2103078 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT171728 NZ_CP078069:c457333-457022 [Comamonas sp. NLF-1-9]
ATGAAGAAGCCCGAAAAAATCAAGATCTCTGACCTGCCCGTGTTCGATGCTGCGGAGTACCTGAAGAGCGAAGAGGACAT
TGCCATTTACCTCACGCTGGCGATTGAAGAGAACGACCCTGCGGGCCTGGCTGATGCGCTGGGCACAATTGCACGCGCTC
GCGGCATGACCGAGGTGGCGCGGGCTAGCGGGCTGACCCGCGAGGCGCTTTACAAGGCCCTGCGCCCCGGCAGCCATCCG
CGCTTTGACACCATCGCCCGCGTGTGTGGAGCGTTGGGCGTGAAGCTGGTGGCGCAGCCAAGCCGTACTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0A6SPA6

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References