171891

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 612201..613000 Replicon chromosome
Accession NZ_CP060987
Organism Escherichia coli strain EC31

Toxin (Protein)


Gene name yhaV Uniprot ID A0A0A6SPA6
Locus tag H9212_RS02990 Protein ID WP_000347275.1
Coordinates 612201..612665 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag H9212_RS02995 Protein ID WP_001307405.1
Coordinates 612665..613000 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H9212_RS02960 (607202) 607202..607636 - 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -
H9212_RS02965 (607654) 607654..608532 - 879 WP_001295548.1 PTS N-acetylgalactosamine transporter subunit IID -
H9212_RS02970 (608522) 608522..609301 - 780 WP_000406209.1 PTS N-acetylgalactosamine transporter subunit IIC -
H9212_RS02975 (609312) 609312..609785 - 474 WP_001336162.1 PTS N-acetylgalactosamine transporter subunit IIB -
H9212_RS02980 (609808) 609808..611088 - 1281 WP_000681920.1 tagatose-bisphosphate aldolase subunit KbaZ -
H9212_RS02985 (611337) 611337..612146 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
H9212_RS02990 (612201) 612201..612665 - 465 WP_000347275.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
H9212_RS02995 (612665) 612665..613000 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
H9212_RS03000 (613149) 613149..614720 - 1572 WP_001273752.1 galactarate dehydratase -
H9212_RS03005 (615095) 615095..616429 + 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
H9212_RS03010 (616445) 616445..617215 + 771 WP_001058209.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 612201..623875 11674


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17820.29 Da        Isoelectric Point: 9.8492

>T171891 WP_000347275.1 NZ_CP060987:c612665-612201 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKVPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

>T171891 NZ_CP078162:c2485793-2485650 [Enterococcus faecalis]
ATGAGCGTACTTCCAAAATTTACAGAAAGGAGAGGCCTTTTGTCAGCATATGAGACAATTCAGACGATCCTAGGTTTTGG
TATGTTTACCATTGCTTTGATTGCGCTGATTGTTAAATTGCTTAAAAATGACAAGAAAAAATAA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT171891 WP_001307405.1 NZ_CP060987:c613000-612665 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT171891 NZ_CP078162:2485531-2485717 [Enterococcus faecalis]
TGTGCTATAATGAAAACGAAAAGAGAGATATGCTTCAACATACCTCTCTGATGTAGAGCCGTTTAAGACGGTGACCAATT
GTATTATTTAAAAATAACCGTACTGGTCAAAGTAGACGGTTATTTTTTCTTGTCATTTTTAAGCAATTTAACAATCAGCG
CAATCAAAGCAATGGTAAACATACCAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0A6SPA6

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References