186546

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 4089560..4090359 Replicon chromosome
Accession NZ_CP068041
Organism Escherichia coli strain XJW9B277

Toxin (Protein)


Gene name yhaV Uniprot ID V0SSH7
Locus tag JJE78_RS19790 Protein ID WP_000347273.1
Coordinates 4089895..4090359 (+) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag JJE78_RS19785 Protein ID WP_001307405.1
Coordinates 4089560..4089895 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JJE78_RS19770 (4085345) 4085345..4086115 - 771 WP_048218026.1 2-dehydro-3-deoxyglucarate aldolase -
JJE78_RS19775 (4086131) 4086131..4087465 - 1335 WP_001723935.1 galactarate/glucarate/glycerate transporter GarP -
JJE78_RS19780 (4087840) 4087840..4089411 + 1572 WP_001723936.1 galactarate dehydratase -
JJE78_RS19785 (4089560) 4089560..4089895 + 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
JJE78_RS19790 (4089895) 4089895..4090359 + 465 WP_000347273.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
JJE78_RS19795 (4090414) 4090414..4091223 - 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
JJE78_RS19800 (4091472) 4091472..4092752 + 1281 WP_000681903.1 tagatose-bisphosphate aldolase subunit KbaZ -
JJE78_RS19805 (4092775) 4092775..4093248 + 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
JJE78_RS19810 (4093259) 4093259..4094038 + 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
JJE78_RS19815 (4094028) 4094028..4094906 + 879 WP_001300474.1 PTS N-acetylgalactosamine transporter subunit IID -
JJE78_RS19820 (4094924) 4094924..4095358 + 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 4080413..4090359 9946


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17836.25 Da        Isoelectric Point: 9.6924

>T186546 WP_000347273.1 NZ_CP068041:4089895-4090359 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

>T186546 NZ_CP089598:327908-328276 [Klebsiella pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACTGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCGGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACCCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCAGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCAGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGTTTGCGTGGATGA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT186546 WP_001307405.1 NZ_CP068041:4089560-4089895 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT186546 NZ_CP089598:327690-327911 [Klebsiella pneumoniae]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGGCGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SSH7

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References