216832

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 2089430..2090229 Replicon chromosome
Accession NZ_CP083610
Organism Escherichia coli strain EC2648-Vir1

Toxin (Protein)


Gene name yhaV Uniprot ID V0SSH7
Locus tag LAJ48_RS10100 Protein ID WP_000347273.1
Coordinates 2089430..2089894 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag LAJ48_RS10105 Protein ID WP_001307405.1
Coordinates 2089894..2090229 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LAJ48_RS10075 (2085606) 2085606..2086163 - 558 Protein_1962 amidohydrolase family protein -
LAJ48_RS10080 (2086159) 2086159..2086530 - 372 Protein_1963 PTS sugar transporter subunit IIC -
LAJ48_RS10085 (2086541) 2086541..2087014 - 474 WP_001336162.1 PTS N-acetylgalactosamine transporter subunit IIB -
LAJ48_RS10090 (2087037) 2087037..2088317 - 1281 WP_000681920.1 tagatose-bisphosphate aldolase subunit KbaZ -
LAJ48_RS10095 (2088566) 2088566..2089375 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
LAJ48_RS10100 (2089430) 2089430..2089894 - 465 WP_000347273.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
LAJ48_RS10105 (2089894) 2089894..2090229 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
LAJ48_RS10110 (2090378) 2090378..2091949 - 1572 WP_001273753.1 galactarate dehydratase -
LAJ48_RS10115 (2092324) 2092324..2093658 + 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
LAJ48_RS10120 (2093674) 2093674..2094444 + 771 WP_001058209.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 2089430..2101104 11674


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17836.25 Da        Isoelectric Point: 9.6924

>T216832 WP_000347273.1 NZ_CP083610:c2089894-2089430 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

>T216832 NZ_CP113487:109291-109707 [Escherichia coli]
GTGAACAAGATCTATATGCTCGACACGAACATCTGCTCGTTCATCATGCGCGAGCAACCTGAAGCATTGCTTAAGCACCT
GGAGCAGTCGGTGCTGCGCGGTCACCGTATCGTGGTCTCGGCCATCACCTACTCCGAGATGCGCTTCGGTGCCACCGGTC
CGAAGGCCTCGCCACGCCACGTGCAACTGGTTGACGCGTTCTGTGAGAGGCTCGATGCCGTCCTGCCGTGGGACCGCGCC
GCGGTGGATGCGACCACGGAGATTAAGGTGGCACTGCGTCTCGCCGGCACGCCGATCGGTCCGAACGACACGGCGATAGC
CGGACACGCCATCGCTGCCTGTGCCATTCTGGTGACGAATAATGTGAGAGAGTTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT216832 WP_001307405.1 NZ_CP083610:c2090229-2089894 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT216832 NZ_CP113487:109064-109294 [Escherichia coli]
ATGAGAACCGTATCCATTTTTAAAAATGGCAACAACCGTGCTATCCGCCTTCCACGCGATCTGGATTTTGAGGGGGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGCCCCGTCCGGCCGACCTGGGGCTCATTCCTGGAGCTAG
AAAAGGCCGATCCGGACTTTATGACGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGAGTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SSH7

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References