200889

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 2235979..2236778 Replicon chromosome
Accession NZ_CP073981
Organism Escherichia coli strain MB19

Toxin (Protein)


Gene name yhaV Uniprot ID V0SSH7
Locus tag KFU84_RS10755 Protein ID WP_000347273.1
Coordinates 2235979..2236443 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag KFU84_RS10760 Protein ID WP_001307405.1
Coordinates 2236443..2236778 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KFU84_RS10725 (2230980) 2230980..2231414 - 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -
KFU84_RS10730 (2231432) 2231432..2232310 - 879 WP_257182214.1 PTS N-acetylgalactosamine transporter subunit IID -
KFU84_RS10735 (2232300) 2232300..2233079 - 780 WP_000406209.1 PTS N-acetylgalactosamine transporter subunit IIC -
KFU84_RS10740 (2233090) 2233090..2233563 - 474 WP_001422480.1 PTS N-acetylgalactosamine transporter subunit IIB -
KFU84_RS10745 (2233586) 2233586..2234866 - 1281 WP_000681920.1 tagatose-bisphosphate aldolase subunit KbaZ -
KFU84_RS10750 (2235115) 2235115..2235924 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
KFU84_RS10755 (2235979) 2235979..2236443 - 465 WP_000347273.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
KFU84_RS10760 (2236443) 2236443..2236778 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
KFU84_RS10765 (2236927) 2236927..2238498 - 1572 WP_001273753.1 galactarate dehydratase -
KFU84_RS10770 (2238873) 2238873..2240207 + 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
KFU84_RS10775 (2240223) 2240223..2240993 + 771 WP_001058209.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 2235979..2247653 11674


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17836.25 Da        Isoelectric Point: 9.6924

>T200889 WP_000347273.1 NZ_CP073981:c2236443-2235979 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

>T200889 NZ_CP098195:449805-450332 [Escherichia coli]
ATGGATGATCTGACGATAGAGATTCTGACCGATGATGCAGATTATGATCTACAGCGATTCGACTGCGGCGAGGAAGCGTT
AAATCTCTTTCTGACGACACATCTCGTTCGTCAACATCGCAACAAAATTCTGCGAGCGTATATCCTTTGTCGCAACACTC
CAGAACGTCAGGTGCTGGGATATTACACATTATGCGGCAGTTGTTTTGAACGAGCCGCATTGCCCTCGAAATCGAAACAG
AAAAAAATTCCCTACAAAAATATTCCCAGCGTTACTCTTGGGCGTCTGGCAATTGATCGTTCATTACAGGGGCAGGGATG
GGGAGCAACACTGGTTGCTCATGCCATGAAAGTCGTCTGGTCAGCCTCTTTAGCGGTAGGTATTCACGGTCTTTTTGTCG
AGGCGCTGAATAAAAAAGCCCATACGTTTTATCAATCGCTGGGCTTTATCCCGTTGGTCGGAGAAAACGAAAATGCGTTA
TTTTTCCCAACCAAATCCATCGAACTGCTTTTCACACAGAGCGATTAA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT200889 WP_001307405.1 NZ_CP073981:c2236778-2236443 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT200889 NZ_CP098195:449532-449798 [Escherichia coli]
ATGTCTGCTGTTAAAAAGCAGCGTATCGATCTGCGTTTAACTGACGATGACAAAAGTATGATTGAGGAAGCGGCAGCGAT
ATCTAATCAGTCCGTCAGCCAATTTATGTTGAACAGCGCTTCGCAGCGGGCTGCGGAAGTGATTGAACAGCATCGGCGGG
TGATCCTCAATGAGGAATCCTGGACGCGGGTGATGGATGCGCTGAGTAATCCACCGTCACCTGGTGAAAAGCTAAAACGT
GCGGCAAAACGTCTTCAGGGAATGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SSH7

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References