Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 2536213..2537012 Replicon chromosome
Accession NZ_AP027411
Organism Escherichia coli strain EC521 isolate EC521

Toxin (Protein)


Gene name yhaV Uniprot ID V0SSH7
Locus tag QUE69_RS12830 Protein ID WP_000347273.1
Coordinates 2536213..2536677 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag QUE69_RS12835 Protein ID WP_001307405.1
Coordinates 2536677..2537012 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QUE69_RS12800 (2531214) 2531214..2531648 - 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -
QUE69_RS12805 (2531666) 2531666..2532544 - 879 WP_001295548.1 PTS N-acetylgalactosamine transporter subunit IID -
QUE69_RS12810 (2532534) 2532534..2533313 - 780 WP_000406209.1 PTS N-acetylgalactosamine transporter subunit IIC -
QUE69_RS12815 (2533324) 2533324..2533797 - 474 WP_001336162.1 PTS N-acetylgalactosamine transporter subunit IIB -
QUE69_RS12820 (2533820) 2533820..2535100 - 1281 WP_000681920.1 tagatose-bisphosphate aldolase subunit KbaZ -
QUE69_RS12825 (2535349) 2535349..2536158 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
QUE69_RS12830 (2536213) 2536213..2536677 - 465 WP_000347273.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
QUE69_RS12835 (2536677) 2536677..2537012 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
QUE69_RS12840 (2537161) 2537161..2538732 - 1572 WP_001273753.1 galactarate dehydratase -
QUE69_RS12845 (2539107) 2539107..2540441 + 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
QUE69_RS12850 (2540457) 2540457..2541227 + 771 WP_001058209.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 2536213..2547888 11675


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17836.25 Da        Isoelectric Point: 9.6924

>T43843 WP_000347273.1 NZ_AP027411:c2536677-2536213 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

>T43843 NZ_AP027411:c2536677-2536213 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT43843 WP_001307405.1 NZ_AP027411:c2537012-2536677 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT43843 NZ_AP027411:c2537012-2536677 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SSH7

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References