216578

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 4264115..4264914 Replicon chromosome
Accession NZ_CP083492
Organism Escherichia coli strain elppa8

Toxin (Protein)


Gene name yhaV Uniprot ID A0A0A6SPA6
Locus tag K9U11_RS20590 Protein ID WP_000347275.1
Coordinates 4264115..4264579 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag K9U11_RS20595 Protein ID WP_001307405.1
Coordinates 4264579..4264914 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K9U11_RS20560 (4259116) 4259116..4259550 - 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -
K9U11_RS20565 (4259568) 4259568..4260446 - 879 WP_001295548.1 PTS N-acetylgalactosamine transporter subunit IID -
K9U11_RS20570 (4260436) 4260436..4261215 - 780 WP_000406209.1 PTS N-acetylgalactosamine transporter subunit IIC -
K9U11_RS20575 (4261226) 4261226..4261699 - 474 WP_001336162.1 PTS N-acetylgalactosamine transporter subunit IIB -
K9U11_RS20580 (4261722) 4261722..4263002 - 1281 WP_228595429.1 tagatose-bisphosphate aldolase subunit KbaZ -
K9U11_RS20585 (4263251) 4263251..4264060 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
K9U11_RS20590 (4264115) 4264115..4264579 - 465 WP_000347275.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
K9U11_RS20595 (4264579) 4264579..4264914 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
K9U11_RS20600 (4265063) 4265063..4266634 - 1572 WP_001273752.1 galactarate dehydratase -
K9U11_RS20605 (4267009) 4267009..4268343 + 1335 WP_228595430.1 galactarate/glucarate/glycerate transporter GarP -
K9U11_RS20610 (4268359) 4268359..4269129 + 771 WP_228595431.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 4264115..4275789 11674


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17820.29 Da        Isoelectric Point: 9.8492

>T216578 WP_000347275.1 NZ_CP083492:c4264579-4264115 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKVPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

>T216578 NZ_CP113168:c4678781-4678497 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCAGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGACCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT216578 WP_001307405.1 NZ_CP083492:c4264914-4264579 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT216578 NZ_CP113168:c4679013-4678771 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCAGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0A6SPA6

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References