37743

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 4253478..4254277 Replicon chromosome
Accession NZ_AP023433
Organism Escherichia coli strain M719

Toxin (Protein)


Gene name yhaV Uniprot ID A0A0A6SPA6
Locus tag M2043_RS20505 Protein ID WP_000347275.1
Coordinates 4253813..4254277 (+) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag M2043_RS20500 Protein ID WP_001307405.1
Coordinates 4253478..4253813 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M2043_RS20485 (4249263) 4249263..4250033 - 771 WP_001058209.1 2-dehydro-3-deoxyglucarate aldolase -
M2043_RS20490 (4250049) 4250049..4251383 - 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
M2043_RS20495 (4251758) 4251758..4253329 + 1572 WP_001273752.1 galactarate dehydratase -
M2043_RS20500 (4253478) 4253478..4253813 + 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
M2043_RS20505 (4253813) 4253813..4254277 + 465 WP_000347275.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
M2043_RS20510 (4254332) 4254332..4255141 - 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
M2043_RS20515 (4255390) 4255390..4256670 + 1281 WP_000681920.1 tagatose-bisphosphate aldolase subunit KbaZ -
M2043_RS20520 (4256693) 4256693..4257166 + 474 WP_001336162.1 PTS N-acetylgalactosamine transporter subunit IIB -
M2043_RS20525 (4257177) 4257177..4257956 + 780 WP_000406209.1 PTS N-acetylgalactosamine transporter subunit IIC -
M2043_RS20530 (4257946) 4257946..4258824 + 879 WP_001295548.1 PTS N-acetylgalactosamine transporter subunit IID -
M2043_RS20535 (4258842) 4258842..4259276 + 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 4244330..4254277 9947


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17820.29 Da        Isoelectric Point: 9.8492

>T37743 WP_000347275.1 NZ_AP023433:4253813-4254277 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKVPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

>T37743 NZ_AP023433:4253813-4254277 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGTTCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT37743 WP_001307405.1 NZ_AP023433:4253478-4253813 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT37743 NZ_AP023433:4253478-4253813 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0A6SPA6

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References