192697

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 399550..400349 Replicon chromosome
Accession NZ_CP070026
Organism Escherichia coli strain FDAARGOS_1337

Toxin (Protein)


Gene name yhaV Uniprot ID V0SSH7
Locus tag I6K67_RS02235 Protein ID WP_000347273.1
Coordinates 399885..400349 (+) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag I6K67_RS02230 Protein ID WP_001307405.1
Coordinates 399550..399885 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6K67_RS02215 (395335) 395335..396105 - 771 WP_001058209.1 2-dehydro-3-deoxyglucarate aldolase -
I6K67_RS02220 (396121) 396121..397455 - 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
I6K67_RS02225 (397830) 397830..399401 + 1572 WP_001273753.1 galactarate dehydratase -
I6K67_RS02230 (399550) 399550..399885 + 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
I6K67_RS02235 (399885) 399885..400349 + 465 WP_000347273.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
I6K67_RS02240 (400404) 400404..401213 - 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
I6K67_RS02245 (401462) 401462..402742 + 1281 WP_000681920.1 tagatose-bisphosphate aldolase subunit KbaZ -
I6K67_RS02250 (402765) 402765..403238 + 474 WP_001336162.1 PTS N-acetylgalactosamine transporter subunit IIB -
I6K67_RS02255 (403249) 403249..404028 + 780 WP_000406209.1 PTS N-acetylgalactosamine transporter subunit IIC -
I6K67_RS02260 (404018) 404018..404896 + 879 WP_001295548.1 PTS N-acetylgalactosamine transporter subunit IID -
I6K67_RS02265 (404914) 404914..405348 + 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 390402..400349 9947


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17836.25 Da        Isoelectric Point: 9.6924

>T192697 WP_000347273.1 NZ_CP070026:399885-400349 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

>T192697 NZ_CP092532:1898179-1898430 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT192697 WP_001307405.1 NZ_CP070026:399550-399885 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT192697 NZ_CP092532:1898427-1898681 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAAATTATCAAAGATACACGCAGAACACCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GCTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SSH7

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References