158981

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 4155752..4156551 Replicon chromosome
Accession NZ_CP054214
Organism Escherichia coli strain EcPF40

Toxin (Protein)


Gene name yhaV Uniprot ID A0A0A6SPA6
Locus tag HR076_RS20145 Protein ID WP_000347275.1
Coordinates 4156087..4156551 (+) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag HR076_RS20140 Protein ID WP_001307405.1
Coordinates 4155752..4156087 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HR076_RS20125 4151537..4152307 - 771 WP_001058209.1 2-dehydro-3-deoxyglucarate aldolase -
HR076_RS20130 4152323..4153657 - 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
HR076_RS20135 4154032..4155603 + 1572 WP_001273752.1 galactarate dehydratase -
HR076_RS20140 4155752..4156087 + 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
HR076_RS20145 4156087..4156551 + 465 WP_000347275.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
HR076_RS20150 4156606..4157415 - 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
HR076_RS20155 4157664..4158944 + 1281 WP_000681933.1 tagatose-bisphosphate aldolase subunit KbaZ -
HR076_RS20160 4158967..4159440 + 474 WP_001336162.1 PTS N-acetylgalactosamine transporter subunit IIB -
HR076_RS20165 4159451..4160230 + 780 WP_000406209.1 PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC -
HR076_RS20170 4160220..4161098 + 879 WP_001295548.1 PTS system mannose/fructose/sorbose family transporter subunit IID -
HR076_RS20175 4161116..4161550 + 435 WP_000948824.1 PTS sugar transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 4146604..4156551 9947


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17820.29 Da        Isoelectric Point: 9.8492

>T158981 WP_000347275.1 NZ_CP054214:4156087-4156551 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKVPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

>T158981 NZ_CP069907:c5066855-5066637 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACCTCTGTCTGGAAATTTATCCGCTAA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT158981 WP_001307405.1 NZ_CP054214:4155752-4156087 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT158981 NZ_CP069907:c5067256-5066882 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0A6SPA6

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References