272551

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 2880416..2881215 Replicon chromosome
Accession NZ_CP117962
Organism Escherichia coli strain JM109

Toxin (Protein)


Gene name yhaV Uniprot ID V0SSH7
Locus tag PT299_RS15195 Protein ID WP_000347273.1
Coordinates 2880416..2880880 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag PT299_RS15200 Protein ID WP_001307405.1
Coordinates 2880880..2881215 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PT299_RS15170 (2876592) 2876592..2877149 - 558 Protein_2712 amidohydrolase family protein -
PT299_RS15175 (2877145) 2877145..2877516 - 372 Protein_2713 PTS sugar transporter subunit IIC -
PT299_RS15180 (2877527) 2877527..2878000 - 474 WP_001336162.1 PTS N-acetylgalactosamine transporter subunit IIB -
PT299_RS15185 (2878023) 2878023..2879303 - 1281 WP_000681920.1 tagatose-bisphosphate aldolase subunit KbaZ -
PT299_RS15190 (2879552) 2879552..2880361 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
PT299_RS15195 (2880416) 2880416..2880880 - 465 WP_000347273.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
PT299_RS15200 (2880880) 2880880..2881215 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
PT299_RS15205 (2881364) 2881364..2882935 - 1572 WP_001273747.1 galactarate dehydratase -
PT299_RS15210 (2883310) 2883310..2884644 + 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
PT299_RS15215 (2884660) 2884660..2885430 + 771 WP_001058209.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 2880416..2892090 11674


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17836.25 Da        Isoelectric Point: 9.6924

>T272551 WP_000347273.1 NZ_CP117962:c2880880-2880416 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT272551 WP_001307405.1 NZ_CP117962:c2881215-2880880 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SSH7

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References