54077

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 3252705..3253504 Replicon chromosome
Accession NZ_CP011342
Organism Escherichia coli K-12 substr. GM4792

Toxin (Protein)


Gene name yhaV Uniprot ID V0SSH7
Locus tag U068_RS16125 Protein ID WP_000347273.1
Coordinates 3253040..3253504 (+) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag U068_RS16120 Protein ID WP_001307405.1
Coordinates 3252705..3253040 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
U068_RS16105 3248490..3249260 - 771 WP_001058209.1 2-dehydro-3-deoxyglucarate aldolase -
U068_RS16110 3249276..3250610 - 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
U068_RS16115 3250985..3252556 + 1572 WP_001273753.1 galactarate dehydratase -
U068_RS16120 3252705..3253040 + 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
U068_RS16125 3253040..3253504 + 465 WP_000347273.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
U068_RS16130 3253559..3254368 - 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
U068_RS16135 3254617..3255897 + 1281 WP_000681920.1 tagatose-bisphosphate aldolase subunit KbaZ -
U068_RS16140 3255920..3256393 + 474 WP_001336162.1 PTS N-acetylgalactosamine transporter subunit IIB -
U068_RS16145 3256404..3256775 + 372 Protein_3087 PTS sugar transporter subunit IIC -
U068_RS16150 3256771..3257328 + 558 Protein_3088 amidohydrolase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 3243670..3253504 9834


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17836.25 Da        Isoelectric Point: 9.6924

>T54077 WP_000347273.1 NZ_CP011342:3253040-3253504 [Escherichia coli K-12]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

>T54077 NZ_CP011342:3253040-3253504 [Escherichia coli K-12]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT54077 WP_001307405.1 NZ_CP011342:3252705-3253040 [Escherichia coli K-12]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT54077 NZ_CP011342:3252705-3253040 [Escherichia coli K-12]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SSH7

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References