143397

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 611199..611998 Replicon chromosome
Accession NZ_CP046025
Organism Escherichia coli strain C439

Toxin (Protein)


Gene name yhaV Uniprot ID V0SSH7
Locus tag GJH08_RS02945 Protein ID WP_000347273.1
Coordinates 611199..611663 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag GJH08_RS02950 Protein ID WP_001307405.1
Coordinates 611663..611998 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GJH08_RS02920 607375..607932 - 558 Protein_571 amidohydrolase family protein -
GJH08_RS02925 607928..608299 - 372 Protein_572 PTS sugar transporter subunit IIC -
GJH08_RS02930 608310..608783 - 474 WP_001336162.1 PTS N-acetylgalactosamine transporter subunit IIB -
GJH08_RS02935 608806..610086 - 1281 WP_000681920.1 tagatose-bisphosphate aldolase subunit KbaZ -
GJH08_RS02940 610335..611144 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
GJH08_RS02945 611199..611663 - 465 WP_000347273.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
GJH08_RS02950 611663..611998 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
GJH08_RS02955 612147..613718 - 1572 WP_001273753.1 galactarate dehydratase -
GJH08_RS02960 614093..615427 + 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
GJH08_RS02965 615443..616213 + 771 WP_001058209.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 611199..622873 11674


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17836.25 Da        Isoelectric Point: 9.6924

>T143397 WP_000347273.1 NZ_CP046025:c611663-611199 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

>T143397 NZ_CP061080:4443783-4444172 [Mesirhizobium sp. NIBR3]
ATGATCGTCGATGCTTCGGCGATTCTGGCCGTTCTGTTCGAGGAACCGGAGAAGGACGGCTTCAGAGACCTGATGCTCAG
GCAGAAAGAGCTTGCCATGTCGCCGGTCAACTATCTCGAGGCTGCCGTCCGGGCCGATGACAAGCGGCATCCGAGAAAAG
GCGTGGAACTGGACGCGCTGCTGCAAGGATTCGGAATCGCGATCACCGTCGTCGATTCCCGGCAGGCCTATCTCGCACGC
GAGGCCTACCAGCGCTTCGGCAAGGGCAACCACCCCGCCCGCCTCAACCTCGGCGACTGCTTCGCCTATGCGCTCGCAAA
GGCCCGCCGAGAGCCGCTTCTCTTCAAGGGCGACGATTTCCGCCAGACCGACGTCGAGGCCGCGTTTTGA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT143397 WP_001307405.1 NZ_CP046025:c611998-611663 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT143397 NZ_CP061080:4443538-4443786 [Mesirhizobium sp. NIBR3]
GTGGGCATGAACATCAAGGACAAGGAGGCTCATCACCTGGCCCGGATGATCGCCGACTTCACCGGTGAATCCCTCACGGC
GGCAGTCGTGGTGGCCTTGCGCGAGAGGCTGGAACGATTGGAGCGGCAGGCTGACATCGCGAAGCGCCGCGCCCTGATCG
ACGAGATCATTCGTCGCTCGGGGCCGACCGCGCCCGGTCTGTCGAGCGATCATTCCGACCTCTACGACGAGGCGGGATTG
CCGAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SSH7

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References