227670

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 640622..641421 Replicon chromosome
Accession NZ_CP089142
Organism Escherichia coli strain XD35

Toxin (Protein)


Gene name yhaV Uniprot ID V0SSH7
Locus tag K0768_RS06475 Protein ID WP_000347273.1
Coordinates 640622..641086 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag K0768_RS06480 Protein ID WP_001307405.1
Coordinates 641086..641421 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K0768_RS06445 (635623) 635623..636057 - 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -
K0768_RS06450 (636075) 636075..636953 - 879 WP_001295548.1 PTS N-acetylgalactosamine transporter subunit IID -
K0768_RS06455 (636943) 636943..637722 - 780 WP_000406209.1 PTS N-acetylgalactosamine transporter subunit IIC -
K0768_RS06460 (637733) 637733..638206 - 474 WP_001336162.1 PTS N-acetylgalactosamine transporter subunit IIB -
K0768_RS06465 (638229) 638229..639509 - 1281 WP_000681920.1 tagatose-bisphosphate aldolase subunit KbaZ -
K0768_RS06470 (639758) 639758..640567 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
K0768_RS06475 (640622) 640622..641086 - 465 WP_000347273.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
K0768_RS06480 (641086) 641086..641421 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
K0768_RS06485 (641570) 641570..643141 - 1572 WP_063084662.1 galactarate dehydratase -
K0768_RS06490 (643516) 643516..644850 + 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
K0768_RS06495 (644866) 644866..645636 + 771 WP_001058209.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 640622..653079 12457


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17836.25 Da        Isoelectric Point: 9.6924

>T227670 WP_000347273.1 NZ_CP089142:c641086-640622 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

>T227670 NZ_CP123013:c656635-656171 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGATGCTTTAGTTGC
CGAAGTCGAGGCATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCTGCCACAAAGTTATTGGCGGTGGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGCTATCGTCTCTTCTTTCGTTACAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACGCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGTAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAAGCCTCACCCAAGAGACAGAAGAAAACCATTGA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT227670 WP_001307405.1 NZ_CP089142:c641421-641086 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT227670 NZ_CP123013:c656970-656635 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTCTTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SSH7

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References