55900

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 3254912..3255711 Replicon chromosome
Accession NZ_CP012126
Organism Escherichia coli strain DH1Ec104

Toxin (Protein)


Gene name yhaV Uniprot ID V0SSH7
Locus tag ADZ27_RS16025 Protein ID WP_000347273.1
Coordinates 3255247..3255711 (+) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag ADZ27_RS16020 Protein ID WP_001307405.1
Coordinates 3254912..3255247 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ADZ27_RS16005 3250697..3251467 - 771 WP_001058209.1 2-dehydro-3-deoxyglucarate aldolase -
ADZ27_RS16010 3251483..3252817 - 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
ADZ27_RS16015 3253192..3254763 + 1572 WP_001273747.1 galactarate dehydratase -
ADZ27_RS16020 3254912..3255247 + 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
ADZ27_RS16025 3255247..3255711 + 465 WP_000347273.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
ADZ27_RS16030 3255766..3256575 - 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
ADZ27_RS16035 3256824..3258104 + 1281 WP_000681920.1 tagatose-bisphosphate aldolase subunit KbaZ -
ADZ27_RS16040 3258127..3258600 + 474 WP_001336162.1 PTS N-acetylgalactosamine transporter subunit IIB -
ADZ27_RS16045 3258611..3258982 + 372 Protein_3073 PTS sugar transporter subunit IIC -
ADZ27_RS16050 3258978..3259535 + 558 Protein_3074 amidohydrolase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 3245764..3255711 9947


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17836.25 Da        Isoelectric Point: 9.6924

>T55900 WP_000347273.1 NZ_CP012126:3255247-3255711 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

>T55900 NZ_CP012126:3255247-3255711 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT55900 WP_001307405.1 NZ_CP012126:3254912-3255247 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT55900 NZ_CP012126:3254912-3255247 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SSH7

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References