234870

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 2345350..2346149 Replicon chromosome
Accession NZ_CP091839
Organism Escherichia coli strain SWYHX138.261ft

Toxin (Protein)


Gene name yhaV Uniprot ID A0A0A6SPA6
Locus tag L7H22_RS11265 Protein ID WP_000347275.1
Coordinates 2345685..2346149 (+) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag L7H22_RS11260 Protein ID WP_001307405.1
Coordinates 2345350..2345685 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L7H22_RS11245 (2341135) 2341135..2341905 - 771 WP_238058944.1 2-dehydro-3-deoxyglucarate aldolase -
L7H22_RS11250 (2341921) 2341921..2343255 - 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
L7H22_RS11255 (2343630) 2343630..2345201 + 1572 WP_001273753.1 galactarate dehydratase -
L7H22_RS11260 (2345350) 2345350..2345685 + 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
L7H22_RS11265 (2345685) 2345685..2346149 + 465 WP_000347275.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
L7H22_RS11270 (2346204) 2346204..2347013 - 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
L7H22_RS11275 (2347262) 2347262..2348542 + 1281 WP_000681920.1 tagatose-bisphosphate aldolase subunit KbaZ -
L7H22_RS11280 (2348565) 2348565..2349038 + 474 WP_001336162.1 PTS N-acetylgalactosamine transporter subunit IIB -
L7H22_RS11285 (2349049) 2349049..2349828 + 780 WP_000406209.1 PTS N-acetylgalactosamine transporter subunit IIC -
L7H22_RS11290 (2349818) 2349818..2350696 + 879 WP_001295548.1 PTS N-acetylgalactosamine transporter subunit IID -
L7H22_RS11295 (2350714) 2350714..2351148 + 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 2336315..2346149 9834


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17820.29 Da        Isoelectric Point: 9.8492

>T234870 WP_000347275.1 NZ_CP091839:2345685-2346149 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKVPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

>T234870 NZ_LR134093:c2180238-2180029 [Staphylococcus aureus]
ATGATACATCAAAATACGATTTACACAGCGGGAATTGAAACAGAAGAACAAGTAAGTCAATTGACAGAACGCATTTCAAA
TATGATAGGTGTTCATCAAGTGATTATTAATATAATAGACGGTCAGGTAACTGTAGCGTATGAGACACCAGCAAATTTGA
ATAGTATTGAAAAAGAAATCTATGATGAAGGATACAAAATTGTATTTTAG

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT234870 WP_001307405.1 NZ_CP091839:2345350-2345685 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT234870 NZ_LR134093:c2180543-2180250 [Staphylococcus aureus]
ATGACTGAACAAGATAATGCACATCATTCTGAACAAATAAAAACGAATCTTAAATCACGTTTAAATCGAATTGAAGGACA
AGTGAGAGCGATTAATCGCATGATTGAAGAAGATGTCTATTGTGATGATGTCCTTACGCAAATAAGAGCGACACGTTCGG
CGTTAAACAGTGTTGCGACAAAGTTATTAGAACAACATATGAAAAGTTGTATTATGAATAAAGTTAATCAAGGTGCTCAG
GAAGAGGCAATGGAAGAGTTATTAGTGACTTTTCAAAAATTGATTAAAGACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0A6SPA6

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References