167246

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 474459..475258 Replicon chromosome
Accession NZ_CP058719
Organism Escherichia coli strain M159-1

Toxin (Protein)


Gene name yhaV Uniprot ID V0SSH7
Locus tag HZS86_RS02315 Protein ID WP_000347273.1
Coordinates 474794..475258 (+) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag HZS86_RS02310 Protein ID WP_001307405.1
Coordinates 474459..474794 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HZS86_RS02295 (470244) 470244..471014 - 771 WP_001058209.1 2-dehydro-3-deoxyglucarate aldolase -
HZS86_RS02300 (471030) 471030..472364 - 1335 WP_130062351.1 galactarate/glucarate/glycerate transporter GarP -
HZS86_RS02305 (472739) 472739..474310 + 1572 WP_001273753.1 galactarate dehydratase -
HZS86_RS02310 (474459) 474459..474794 + 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
HZS86_RS02315 (474794) 474794..475258 + 465 WP_000347273.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
HZS86_RS02320 (475313) 475313..476122 - 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
HZS86_RS02325 (476371) 476371..477651 + 1281 WP_000681920.1 tagatose-bisphosphate aldolase subunit KbaZ -
HZS86_RS02330 (477674) 477674..478147 + 474 WP_001336162.1 PTS N-acetylgalactosamine transporter subunit IIB -
HZS86_RS02335 (478158) 478158..478937 + 780 WP_000406209.1 PTS N-acetylgalactosamine transporter subunit IIC -
HZS86_RS02340 (478927) 478927..479805 + 879 WP_001295548.1 PTS N-acetylgalactosamine transporter subunit IID -
HZS86_RS02345 (479823) 479823..480257 + 435 WP_000948842.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 464358..475258 10900


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17836.25 Da        Isoelectric Point: 9.6924

>T167246 WP_000347273.1 NZ_CP058719:474794-475258 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

>T167246 NZ_CP075106:3496910-3497128 [Salmonella enterica]
ATGTCTGATAAACCATTAACTAAAACTGATTATTTGATGCGTTTACGGCGCTGTCAGACAATTGACACTCTGGAGCGCGT
CATTGAGAAAAATAAATATGAATTGTCCGACAATGAGCTGGCTGTATTTTACTCAGCTGCGGATCACCGTCTTGCAGAAT
TGACGATGAACAAACTCTACGACAAGATCCCCTCTTCAGTATGGAAATTCATTCGTTAA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT167246 WP_001307405.1 NZ_CP058719:474459-474794 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT167246 NZ_CP075106:3496508-3496882 [Salmonella enterica]
ATGGACGAATACTCACCCAAAAGACATGATATAGCACAGCTTAAATTTCTTTGCGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAACCATGGCTGGGTTAATGACCCGACCTCAGCAGTCAACCTGCAGCTCAATGAGCTGATAGAGC
ATATTGCAACTTTCGCGCTTAATTATAAAATTAAGTACAATGAAGACAATAAATTGATTGCGCAGATAGATGAATACCTG
GACGACACCTTTATGTTGTTCAGCAGCTATGGTATTAATACGCAGGATTTGCAAAAATGGCGTAAATCGGGCAACCGCCT
GTTCCGTTGTTTCGTCAACGCCACCAGGGCCAATCCTGTTAGTTTGTCTTGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SSH7

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References