Detailed information of TA system TA027464

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 3763348..3764147
• Replicon: chromosome
• Accession: NC_017641
• Organism: Escherichia coli UMNK88

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: V0SSH7
• Locus tag: UMNK88_RS19325
• Protein ID: WP_000347273.1
• Coordinates: 3763683..3764147 (+)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: S1EB98
• Locus tag: UMNK88_RS19320
• Protein ID: WP_001307405.1
• Coordinates: 3763348..3763683 (+)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
UMNK88_RS19305	3759133..3759903	-	771	WP_001058209.1	2-dehydro-3-deoxyglucarate aldolase	-
UMNK88_RS19310	3759919..3761253	-	1335	WP_000599636.1	galactarate/glucarate/glycerate transporter GarP	-
UMNK88_RS19315	3761628..3763199	+	1572	WP_001273754.1	galactarate dehydratase	-
UMNK88_RS19320	3763348..3763683	+	336	WP_001307405.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
UMNK88_RS19325	3763683..3764147	+	465	WP_000347273.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
UMNK88_RS19330	3764202..3765011	-	810	WP_000072187.1	aga operon transcriptional regulator AgaR	-
UMNK88_RS19335	3765260..3766540	+	1281	WP_000681920.1	tagatose-bisphosphate aldolase subunit KbaZ	-
UMNK88_RS19340	3766563..3767036	+	474	WP_001295547.1	PTS N-acetylgalactosamine transporter subunit IIB	-
UMNK88_RS19345	3767047..3767826	+	780	WP_000406214.1	PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC	-
UMNK88_RS19350	3767816..3768694	+	879	WP_001295548.1	PTS system mannose/fructose/sorbose family transporter subunit IID	-
UMNK88_RS19355	3768712..3769146	+	435	WP_000948814.1	PTS sugar transporter subunit IIA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View HG738867 Escherichia coli str. K-12 substr. MC4100 Genomic island TA020080 3504819..3516493 0.007 View NC_000913 Escherichia coli str. K-12 substr. MG1655 Genomic island TA001047 3267854..3277801 0.002 View NC_007779 Escherichia coli str. K-12 substr. W3110 Genomic island TA021455 3265982..3277656 0.007 View NC_010473 Escherichia coli str. K-12 substr. DH10B Genomic island TA022703 3363621..3373568 0.002 View NC_012759 Escherichia coli BW2952 Genomic island TA023871 3153024..3162971 0.002 View NC_017638 Escherichia coli DH1 Genomic island TA027421 3245760..3255707 0.003 View NC_020518 Escherichia coli str. K-12 substr. MDS42 Genomic island TA029016 2741187..2751134 0.002 View NZ_CP009273 Escherichia coli BW25113 Genomic island TA048700 3261213..3271160 0.002 View NZ_CP017979 Escherichia coli str. K-12 substr. W3110 strain K-12 substr. ZK126 Genomic island TA068442 3158126..3169800 0.007 View NZ_CP020543 Escherichia coli C Genomic island TA075057 640607..652281 0.007 View NZ_CP025520 Escherichia coli strain DH5alpha Genomic island TA090495 2121277..2131224 0.003 View NZ_HG738867 Escherichia coli str. K-12 substr. MC4100 strain K-12 Genomic island TA285025 3504819..3516493 0.007	inside	Genomic island	-	-	3754873..3764147	9274

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17836.25 Da
• Isoelectric Point: 9.6924

>T27464 WP_000347273.1 NC_017641:3763683-3764147 [Escherichia coli UMNK88]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Nucleotide

• Length: 465 bp

>T27464 NC_017641:3763683-3764147 [Escherichia coli UMNK88]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12358.94 Da
• Isoelectric Point: 4.8616

>AT27464 WP_001307405.1 NC_017641:3763348-3763683 [Escherichia coli UMNK88]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT27464 NC_017641:3763348-3763683 [Escherichia coli UMNK88]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Structures

Toxin

• AlphaFold DB: V0SSH7

Antitoxin

• AlphaFold DB: A0A0E0XVC7