257433

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 618403..619202 Replicon chromosome
Accession NZ_CP104274
Organism Escherichia coli strain Ec15103

Toxin (Protein)


Gene name yhaV Uniprot ID A0A0A6SPA6
Locus tag N2O69_RS02990 Protein ID WP_000347275.1
Coordinates 618403..618867 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag N2O69_RS02995 Protein ID WP_001307405.1
Coordinates 618867..619202 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N2O69_RS02960 (613404) 613404..613838 - 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -
N2O69_RS02965 (613856) 613856..614734 - 879 WP_001295548.1 PTS N-acetylgalactosamine transporter subunit IID -
N2O69_RS02970 (614724) 614724..615503 - 780 WP_000406209.1 PTS N-acetylgalactosamine transporter subunit IIC -
N2O69_RS02975 (615514) 615514..615987 - 474 WP_001336162.1 PTS N-acetylgalactosamine transporter subunit IIB -
N2O69_RS02980 (616010) 616010..617290 - 1281 WP_000681933.1 tagatose-bisphosphate aldolase subunit KbaZ -
N2O69_RS02985 (617539) 617539..618348 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
N2O69_RS02990 (618403) 618403..618867 - 465 WP_000347275.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
N2O69_RS02995 (618867) 618867..619202 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
N2O69_RS03000 (619351) 619351..620922 - 1572 WP_001273752.1 galactarate dehydratase -
N2O69_RS03005 (621297) 621297..622631 + 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
N2O69_RS03010 (622647) 622647..623417 + 771 WP_001058209.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 618403..630077 11674


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17820.29 Da        Isoelectric Point: 9.8492

>T257433 WP_000347275.1 NZ_CP104274:c618867-618403 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKVPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT257433 WP_001307405.1 NZ_CP104274:c619202-618867 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0A6SPA6

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References