198072

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 642154..642953 Replicon chromosome
Accession NZ_CP072565
Organism Escherichia coli strain WG5::PA8

Toxin (Protein)


Gene name yhaV Uniprot ID V0SSH7
Locus tag J8F86_RS03070 Protein ID WP_000347273.1
Coordinates 642154..642618 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag J8F86_RS03075 Protein ID WP_001307405.1
Coordinates 642618..642953 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J8F86_RS03040 (637155) 637155..637589 - 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -
J8F86_RS03045 (637607) 637607..638485 - 879 WP_001295548.1 PTS N-acetylgalactosamine transporter subunit IID -
J8F86_RS03050 (638475) 638475..639254 - 780 WP_000406209.1 PTS N-acetylgalactosamine transporter subunit IIC -
J8F86_RS03055 (639265) 639265..639738 - 474 WP_081508768.1 PTS N-acetylgalactosamine transporter subunit IIB -
J8F86_RS03060 (639761) 639761..641041 - 1281 WP_000681920.1 tagatose-bisphosphate aldolase subunit KbaZ -
J8F86_RS03065 (641290) 641290..642099 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
J8F86_RS03070 (642154) 642154..642618 - 465 WP_000347273.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
J8F86_RS03075 (642618) 642618..642953 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
J8F86_RS03080 (643102) 643102..644673 - 1572 WP_001273753.1 galactarate dehydratase -
J8F86_RS03085 (645048) 645048..646382 + 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
J8F86_RS03090 (646398) 646398..647168 + 771 WP_001058209.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 642154..653828 11674


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17836.25 Da        Isoelectric Point: 9.6924

>T198072 WP_000347273.1 NZ_CP072565:c642618-642154 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

>T198072 NZ_CP096840:c3068279-3067923 [Mycobacterium tuberculosis variant bovis]
GTGATGCGCCGCGGTGAGATTTGGCAGGTCGATCTCGACCCCGCTCGAGGTAGCGAAGCGAACAACCAGCGCCCCGCCGT
CGTCGTCAGCAACGACCGGGCCAACGCGACCGCCACGCGTCTTGGGCGCGGCGTCATCACCGTCGTGCCGGTGACGAGCA
ACATCGCCAAGGTCTATCCGTTTCAGGTGTTGTTGTCGGCCACCACTACTGGTCTCCAGGTCGACTGCAAGGCGCAGGCC
GAGCAAATCAGATCGATTGCTACCGAGCGGTTGCTCCGGCCAATCGGCCGAGTTTCAGCCGCCGAACTTGCCCAGCTCGA
TGAGGCTTTGAAACTGCATCTCGACTTATGGTCGTAG

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT198072 WP_001307405.1 NZ_CP072565:c642953-642618 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT198072 NZ_CP096840:c3068493-3068263 [Mycobacterium tuberculosis variant bovis]
GTGAAGTTGAGCGTGAGCCTGTCCGATGACGACGTCGCGATCCTGGACGCATATGTGAAACGAGCGGGATTACCATCCCG
CTCCGCCGGCCTGCAGCATGCGATCCGCGTGCTTCGCTACCCCACGCTCGAAGATGACTATGCCAACGCATGGCAAGAAT
GGTCGGCAGCCGGCGACACGGACGCGTGGGAGCAGACCGTCGGCGACGGAGTCGGTGATGCGCCGCGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SSH7

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References