Detailed information of TA system TA136237

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 3975599..3976398
• Replicon: chromosome
• Accession: NZ_CP042846
• Organism: Escherichia coli strain JME64

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: V0SSH7
• Locus tag: FVE20_RS19305
• Protein ID: WP_000347273.1
• Coordinates: 3975934..3976398 (+)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: S1EB98
• Locus tag: FVE20_RS19300
• Protein ID: WP_001307405.1
• Coordinates: 3975599..3975934 (+)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FVE20_RS19285	3971384..3972154	-	771	WP_001058209.1	2-dehydro-3-deoxyglucarate aldolase	-
FVE20_RS19290	3972170..3973504	-	1335	WP_000599636.1	galactarate/glucarate/glycerate transporter GarP	-
FVE20_RS19295	3973879..3975450	+	1572	WP_001273753.1	galactarate dehydratase	-
FVE20_RS19300	3975599..3975934	+	336	WP_001307405.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
FVE20_RS19305	3975934..3976398	+	465	WP_000347273.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
FVE20_RS19310	3976453..3977262	-	810	WP_000072187.1	aga operon transcriptional regulator AgaR	-
FVE20_RS19315	3977511..3978791	+	1281	WP_000681920.1	tagatose-bisphosphate aldolase subunit KbaZ	-
FVE20_RS19320	3978814..3979287	+	474	WP_001336162.1	PTS N-acetylgalactosamine transporter subunit IIB	-
FVE20_RS19325	3979298..3979669	+	372	Protein_3727	PTS sugar transporter subunit IIC	-
FVE20_RS19330	3979665..3980222	+	558	Protein_3728	amidohydrolase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View HG738867 Escherichia coli str. K-12 substr. MC4100 Genomic island TA020080 3504819..3516493 0.004 View NC_000913 Escherichia coli str. K-12 substr. MG1655 Genomic island TA001047 3267854..3277801 0 View NC_007779 Escherichia coli str. K-12 substr. W3110 Genomic island TA021455 3265982..3277656 0.004 View NC_009800 Escherichia coli HS Genomic island TA022436 3314965..3326421 0.008 View NC_010473 Escherichia coli str. K-12 substr. DH10B Genomic island TA022703 3363621..3373568 0 View NC_012759 Escherichia coli BW2952 Genomic island TA023871 3153024..3162971 0 View NC_017638 Escherichia coli DH1 Genomic island TA027421 3245760..3255707 0 View NC_020518 Escherichia coli str. K-12 substr. MDS42 Genomic island TA029016 2741187..2751134 0 View NZ_CP009273 Escherichia coli BW25113 Genomic island TA048700 3261213..3271160 0 View NZ_CP017979 Escherichia coli str. K-12 substr. W3110 strain K-12 substr. ZK126 Genomic island TA068442 3158126..3169800 0.004 View NZ_CP020543 Escherichia coli C Genomic island TA075057 640607..652281 0.005 View NZ_CP025520 Escherichia coli strain DH5alpha Genomic island TA090495 2121277..2131224 0 View NZ_HG738867 Escherichia coli str. K-12 substr. MC4100 strain K-12 Genomic island TA285025 3504819..3516493 0.004	inside	Genomic island	-	-	3966451..3976398	9947

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17836.25 Da
• Isoelectric Point: 9.6924

>T136237 WP_000347273.1 NZ_CP042846:3975934-3976398 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Nucleotide

• Length: 465 bp

>T136237 NZ_CP055293:c4858350-4857433 [Klebsiella pneumoniae]
ATGGATATACAGGCGCCTTATTATCGGCAGGTTGCTCTGCTAATGCAGGTTTTACCCTATGTTGCTGTCGAACGGGAATT
TGCGCTGAAAGGTGGCACTGCCATCAATCTCTTTATCCGCGATTTCCCTCGATTATCGGTGGATATCGACCTCGCCTGGG
TGCCCCTGGAAAGCAGAGCCATCGCTTTACCTCATATACGGGATGCGCTTGCACGTATTGCCGCCAATCTGCAACAACAG
GCAGGTATGAGCGCTGTATTACAAGCTAATCGTTCAGATGAGATGCGTGTCATTGTTACTACCGATAGCGCGCAAATAAA
GATAGAGGTTTCACCGGTCGCCCGGGGAACATTATATCCACCACAGGAGCGAGAAGTGGTGGGGGCGGTAGAGGACGTTT
TTGGCTATGCCAGCCTTCCAGTGGTTTCGTTGCCTGACCTCTACGGTGGAAAATTATGCGCGGCGCTGGATCGCCAGCAT
CCCAGAGATTTTTATGACGTGAAATTACTTCTTGATGCGCAGGAGCTTGACCGTCCCATTTTCAATGGGTTTATCGTGTA
TTTACTGAGCCATAATCGGCCTCTGGCTGAAGTGCTGAATCCTCGCTGGAAAGATATTGCTGAACCTTTTTACCGTGAAT
TCAGCGGGATGACATTTGAGACGATAGCCCTGGAGGAACTGACAGCAGTCCCCAACAGGATGATTGCTGCGTTAAAATCC
TGTTTCACACAACAGGATGTCGATTTTTTACTGTCATTTAAACGAGGCGAACCAGACTGGCGTTTAGCGCCGGAAATGCG
TATTCAGGATTTGCCTGCCGTGCAGTGGAAATTGCGCAATATTCATCAGATGCCAGCGATAAAACGCGCTGAGTCATTAG
ATAAACTGGAAAAGGTGCTGGCCGAGTGGCGCAGTTAG

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12358.94 Da
• Isoelectric Point: 4.8616

>AT136237 WP_001307405.1 NZ_CP042846:3975599-3975934 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT136237 NZ_CP042846:3975599-3975934 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Structures

Toxin

• AlphaFold DB: V0SSH7

Antitoxin

• AlphaFold DB: A0A0E0XVC7