216388

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 3276863..3277662 Replicon chromosome
Accession NZ_CP083410
Organism Escherichia coli strain RP437

Toxin (Protein)


Gene name yhaV Uniprot ID V0SSH7
Locus tag LAJ62_RS15980 Protein ID WP_000347273.1
Coordinates 3277198..3277662 (+) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag LAJ62_RS15975 Protein ID WP_001307405.1
Coordinates 3276863..3277198 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LAJ62_RS15960 (3272648) 3272648..3273418 - 771 WP_001058209.1 2-dehydro-3-deoxyglucarate aldolase -
LAJ62_RS15965 (3273434) 3273434..3274768 - 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
LAJ62_RS15970 (3275143) 3275143..3276714 + 1572 WP_001273753.1 galactarate dehydratase -
LAJ62_RS15975 (3276863) 3276863..3277198 + 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
LAJ62_RS15980 (3277198) 3277198..3277662 + 465 WP_000347273.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
LAJ62_RS15985 (3277717) 3277717..3278526 - 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
LAJ62_RS15990 (3278775) 3278775..3280055 + 1281 WP_001698595.1 tagatose-bisphosphate aldolase subunit KbaZ -
LAJ62_RS15995 (3280078) 3280078..3280551 + 474 WP_001336162.1 PTS N-acetylgalactosamine transporter subunit IIB -
LAJ62_RS16000 (3280562) 3280562..3280933 + 372 Protein_3127 PTS sugar transporter subunit IIC -
LAJ62_RS16005 (3280929) 3280929..3281486 + 558 Protein_3128 amidohydrolase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 3267715..3277662 9947


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17836.25 Da        Isoelectric Point: 9.6924

>T216388 WP_000347273.1 NZ_CP083410:3277198-3277662 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

>T216388 NZ_CP113037:768845-769111 [Staphylococcus aureus]
ATGGCTAGGTTAAATATTACGTTTTCGCCTCAAGCCTTTGAAGATTATAAGTATTTTCAGCAGAACGATAAAAAAATGGT
GAAGAAGATTAATGAGTTACTTAAAAGTATTGACAGAAATGGTGCATTGAAAGGTATAGGTAAGCCTGAAAAGTTAAAAT
CGAATCTGACTGGTTATTATAGTAGACGTATCAATCACGAACATAGATTGGTCTATACAGTAGATGACAATCATATAAAA
ATAGCATCATGTAAATACCATTATTAA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT216388 WP_001307405.1 NZ_CP083410:3276863-3277198 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT216388 NZ_CP113037:768594-768845 [Staphylococcus aureus]
ATGATTATTAAAAATTATTCATACGCTCGACAGAATTTAAAGGCACTTATGACAAAAGTAAATGATGATAGTGATATGGT
AACTGTAACCTCTACTGATGATAAAAACGTAGTAATCATGTCAGAATCAGATTATAACTCCATGATGGAAACACTTTACC
TCCAACAGAATCCAAATAATGCTGAACACTTAGCTCAATCAATTGCAGATCTAGAACGTGGAAAAACTATAACGAAAGAT
ATAGATGTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SSH7

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References