118899

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 4002204..4003003 Replicon chromosome
Accession NZ_CP035865
Organism Escherichia coli strain E275 a

Toxin (Protein)


Gene name yhaV Uniprot ID V0SSH7
Locus tag EXE34_RS19690 Protein ID WP_000347273.1
Coordinates 4002539..4003003 (+) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag EXE34_RS19685 Protein ID WP_001307405.1
Coordinates 4002204..4002539 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EXE34_RS19670 (3997989) 3997989..3998759 - 771 WP_001058209.1 2-dehydro-3-deoxyglucarate aldolase -
EXE34_RS19675 (3998775) 3998775..4000109 - 1335 WP_159382447.1 galactarate/glucarate/glycerate transporter GarP -
EXE34_RS19680 (4000484) 4000484..4002055 + 1572 WP_001273753.1 galactarate dehydratase -
EXE34_RS19685 (4002204) 4002204..4002539 + 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
EXE34_RS19690 (4002539) 4002539..4003003 + 465 WP_000347273.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
EXE34_RS19695 (4003058) 4003058..4003867 - 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
EXE34_RS19700 (4004116) 4004116..4005396 + 1281 WP_000681920.1 tagatose-bisphosphate aldolase subunit KbaZ -
EXE34_RS19705 (4005419) 4005419..4005892 + 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
EXE34_RS19710 (4005903) 4005903..4006682 + 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
EXE34_RS19715 (4006672) 4006672..4007550 + 879 WP_001295548.1 PTS N-acetylgalactosamine transporter subunit IID -
EXE34_RS19720 (4007568) 4007568..4008002 + 435 WP_000948814.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 3993056..4003003 9947


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17836.25 Da        Isoelectric Point: 9.6924

>T118899 WP_000347273.1 NZ_CP035865:4002539-4003003 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

>T118899 NZ_CP035865:4002539-4003003 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT118899 WP_001307405.1 NZ_CP035865:4002204-4002539 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT118899 NZ_CP035865:4002204-4002539 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SSH7

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References