236424

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 618343..619142 Replicon chromosome
Accession NZ_CP092534
Organism Escherichia coli strain DS566

Toxin (Protein)


Gene name yhaV Uniprot ID A0A0A6SPA6
Locus tag MKD44_RS02990 Protein ID WP_000347275.1
Coordinates 618343..618807 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag MKD44_RS02995 Protein ID WP_001307405.1
Coordinates 618807..619142 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MKD44_RS02960 (613344) 613344..613778 - 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -
MKD44_RS02965 (613796) 613796..614674 - 879 WP_001295548.1 PTS N-acetylgalactosamine transporter subunit IID -
MKD44_RS02970 (614664) 614664..615443 - 780 WP_000406209.1 PTS N-acetylgalactosamine transporter subunit IIC -
MKD44_RS02975 (615454) 615454..615927 - 474 WP_001336162.1 PTS N-acetylgalactosamine transporter subunit IIB -
MKD44_RS02980 (615950) 615950..617230 - 1281 WP_000681933.1 tagatose-bisphosphate aldolase subunit KbaZ -
MKD44_RS02985 (617479) 617479..618288 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
MKD44_RS02990 (618343) 618343..618807 - 465 WP_000347275.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
MKD44_RS02995 (618807) 618807..619142 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
MKD44_RS03000 (619291) 619291..620862 - 1572 WP_001273752.1 galactarate dehydratase -
MKD44_RS03005 (621237) 621237..622571 + 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
MKD44_RS03010 (622587) 622587..623357 + 771 WP_001058209.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 618343..630017 11674


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17820.29 Da        Isoelectric Point: 9.8492

>T236424 WP_000347275.1 NZ_CP092534:c618807-618343 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKVPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

>T236424 NZ_LR594037:1492204-1492482 [Streptococcus anginosus]
ATGTACCAAGTCAAGTTCACCTCTGCTTTTAAAAAGAGCTACAAACGGATTAAAAAGCGAGGCTTAGATATAGCCCTTCT
AGATGAAGTGATTGAAAAACTCCGTCTAGGACAGACACTAGAAGAAAAATACCGCGACCACGAACTCAAAGGAAATTTTG
TTGGTTTTAGGGAATGTCATATTAAGCCAGATTGGCTCTTGATTTATCTCATTGAAGATGATATTTTGACATTAACTTTA
GCTGATACAGGCAGTCATGCGGATTTGTTTAAGATGTGA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT236424 WP_001307405.1 NZ_CP092534:c619142-618807 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT236424 NZ_LR594037:1491931-1492203 [Streptococcus anginosus]
ATGGCAACTGTACCAACGCAAATTCGGATTGATGAAACTGTCAAAGCCCAAGCAACTTCATTATTTAATGACTTGGGGAT
GGATATGTCTAGTGCGGTTAATATTTTTCTTCGTCAATGTCTATTGCGTGGTGGGCTTCCTTTCACGGTTGAAGTTCCCA
ATTATAGTCAAAAGACCTTAGAAGCTCTGGCAGAAGCTAAGCGTATTTCTCGTGATGAAGCGGTCGCTTCTTATGACAAT
CTAGCAGATTTAAAGAAGGCTTTGGAAGCTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0A6SPA6

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References