223537

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 611445..612244 Replicon chromosome
Accession NZ_CP086490
Organism Escherichia coli strain Ec-050-T5-ESBL

Toxin (Protein)


Gene name yhaV Uniprot ID A0A0A6SPA6
Locus tag LNN51_RS02975 Protein ID WP_000347275.1
Coordinates 611445..611909 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag LNN51_RS02980 Protein ID WP_001307405.1
Coordinates 611909..612244 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LNN51_RS02945 (606446) 606446..606880 - 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -
LNN51_RS02950 (606898) 606898..607776 - 879 WP_001295548.1 PTS N-acetylgalactosamine transporter subunit IID -
LNN51_RS02955 (607766) 607766..608545 - 780 WP_000406209.1 PTS N-acetylgalactosamine transporter subunit IIC -
LNN51_RS02960 (608556) 608556..609029 - 474 WP_001336162.1 PTS N-acetylgalactosamine transporter subunit IIB -
LNN51_RS02965 (609052) 609052..610332 - 1281 WP_000681920.1 tagatose-bisphosphate aldolase subunit KbaZ -
LNN51_RS02970 (610581) 610581..611390 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
LNN51_RS02975 (611445) 611445..611909 - 465 WP_000347275.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
LNN51_RS02980 (611909) 611909..612244 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
LNN51_RS02985 (612393) 612393..613964 - 1572 WP_001273752.1 galactarate dehydratase -
LNN51_RS02990 (614339) 614339..615673 + 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
LNN51_RS02995 (615689) 615689..616459 + 771 WP_001058209.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 611445..623119 11674


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17820.29 Da        Isoelectric Point: 9.8492

>T223537 WP_000347275.1 NZ_CP086490:c611909-611445 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKVPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

>T223537 NZ_CP118940:151194-151445 [Aeromonas caviae]
ATGGACGCTATCACTTACACACACGCCCGCGCCAACCTGGCTGGAACCATGGATCGTGTTTGCAACGATCATGAGCCGGT
GATCATCACACGTAACGGCGACCAATCGGTTGTGATTCTCTCGCTGGAAGATTTCCAGGCGCTGGAAGAAACTGCGTATC
TGCTGCGCAGCCCCGCCAACGCCAAGCGGCTTTTCAAGTCGATCGAGCAACTTGAAGCGGGCCGAGGACAGGCGCGGGAG
TTGGCCGAATGA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT223537 WP_001307405.1 NZ_CP086490:c612244-611909 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT223537 NZ_CP118940:151442-151696 [Aeromonas caviae]
ATGAGATTAATTTTTTCCGATGATGCATGGGAAGATTATCTGTTCTGGCAAAAACAAGACCGCCGCATGGTTGATCGAAT
TAACAAGCTAATTAAAGAAACCACCCGAGAGCCTTTCTCTGGAGTGGGCAAGCCAGAGCCATTAAAACATGCTTTGGCCG
GTTATTGGTCTCGTCGAATTACGGATGAGCACCGAATGGTTTACAAAGTGGCGGATGATGCCCTTTGGATCGTTCAACTC
AAGTACCACTACTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0A6SPA6

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References