246487

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 1231063..1231862 Replicon chromosome
Accession NZ_CP097884
Organism Escherichia coli str. K-12 substr. MG1655 strain K-12

Toxin (Protein)


Gene name yhaV Uniprot ID V0SSH7
Locus tag NAG72_RS05890 Protein ID WP_000347273.1
Coordinates 1231063..1231527 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag NAG72_RS05895 Protein ID WP_001307405.1
Coordinates 1231527..1231862 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NAG72_RS05865 (1227239) 1227239..1227796 - 558 Protein_1128 amidohydrolase family protein -
NAG72_RS05870 (1227792) 1227792..1228163 - 372 Protein_1129 PTS sugar transporter subunit IIC -
NAG72_RS05875 (1228174) 1228174..1228647 - 474 WP_001336162.1 PTS N-acetylgalactosamine transporter subunit IIB -
NAG72_RS05880 (1228670) 1228670..1229950 - 1281 WP_000681920.1 tagatose-bisphosphate aldolase subunit KbaZ -
NAG72_RS05885 (1230199) 1230199..1231008 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
NAG72_RS05890 (1231063) 1231063..1231527 - 465 WP_000347273.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
NAG72_RS05895 (1231527) 1231527..1231862 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
NAG72_RS05900 (1232011) 1232011..1233582 - 1572 WP_001273753.1 galactarate dehydratase -
NAG72_RS05905 (1233957) 1233957..1235291 + 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
NAG72_RS05910 (1235307) 1235307..1236077 + 771 WP_001058209.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 1231063..1242737 11674


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17836.25 Da        Isoelectric Point: 9.6924

>T246487 WP_000347273.1 NZ_CP097884:c1231527-1231063 [Escherichia coli str. K-12 substr. MG1655]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT246487 WP_001307405.1 NZ_CP097884:c1231862-1231527 [Escherichia coli str. K-12 substr. MG1655]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SSH7

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References