257831

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 604511..605310 Replicon chromosome
Accession NZ_CP104384
Organism Escherichia coli strain JNQH498

Toxin (Protein)


Gene name yhaV Uniprot ID V0SSH7
Locus tag KIG00_RS02935 Protein ID WP_000347273.1
Coordinates 604511..604975 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag KIG00_RS02940 Protein ID WP_001307405.1
Coordinates 604975..605310 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KIG00_RS02905 (599512) 599512..599946 - 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -
KIG00_RS02910 (599964) 599964..600842 - 879 WP_001295548.1 PTS N-acetylgalactosamine transporter subunit IID -
KIG00_RS02915 (600832) 600832..601611 - 780 WP_000406209.1 PTS N-acetylgalactosamine transporter subunit IIC -
KIG00_RS02920 (601622) 601622..602095 - 474 WP_001336162.1 PTS N-acetylgalactosamine transporter subunit IIB -
KIG00_RS02925 (602118) 602118..603398 - 1281 WP_000681920.1 tagatose-bisphosphate aldolase subunit KbaZ -
KIG00_RS02930 (603647) 603647..604456 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
KIG00_RS02935 (604511) 604511..604975 - 465 WP_000347273.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
KIG00_RS02940 (604975) 604975..605310 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
KIG00_RS02945 (605459) 605459..607030 - 1572 WP_001273753.1 galactarate dehydratase -
KIG00_RS02950 (607405) 607405..608739 + 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
KIG00_RS02955 (608755) 608755..609525 + 771 WP_001058209.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 604511..616185 11674


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17836.25 Da        Isoelectric Point: 9.6924

>T257831 WP_000347273.1 NZ_CP104384:c604975-604511 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT257831 WP_001307405.1 NZ_CP104384:c605310-604975 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SSH7

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References