277551

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 646187..646986 Replicon chromosome
Accession NZ_CP122655
Organism Escherichia coli strain ETEC4074

Toxin (Protein)


Gene name yhaV Uniprot ID V0SSH7
Locus tag QDW60_RS03145 Protein ID WP_000347273.1
Coordinates 646187..646651 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag QDW60_RS03150 Protein ID WP_001307405.1
Coordinates 646651..646986 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QDW60_RS03115 (641188) 641188..641622 - 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -
QDW60_RS03120 (641640) 641640..642518 - 879 WP_001295548.1 PTS N-acetylgalactosamine transporter subunit IID -
QDW60_RS03125 (642508) 642508..643287 - 780 WP_000406209.1 PTS N-acetylgalactosamine transporter subunit IIC -
QDW60_RS03130 (643298) 643298..643771 - 474 WP_001336162.1 PTS N-acetylgalactosamine transporter subunit IIB -
QDW60_RS03135 (643794) 643794..645074 - 1281 WP_000681920.1 tagatose-bisphosphate aldolase subunit KbaZ -
QDW60_RS03140 (645323) 645323..646132 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
QDW60_RS03145 (646187) 646187..646651 - 465 WP_000347273.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
QDW60_RS03150 (646651) 646651..646986 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
QDW60_RS03155 (647135) 647135..648706 - 1572 WP_001273753.1 galactarate dehydratase -
QDW60_RS03160 (649081) 649081..650415 + 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
QDW60_RS03165 (650431) 650431..651201 + 771 WP_001058209.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 646187..657860 11673


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17836.25 Da        Isoelectric Point: 9.6924

>T277551 WP_000347273.1 NZ_CP122655:c646651-646187 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT277551 WP_001307405.1 NZ_CP122655:c646986-646651 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SSH7

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References