235526

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 2960378..2961177 Replicon chromosome
Accession NZ_CP092037
Organism Escherichia coli strain MG1655-377GD

Toxin (Protein)


Gene name yhaV Uniprot ID V0SSH7
Locus tag MBA16_RS14720 Protein ID WP_000347273.1
Coordinates 2960713..2961177 (+) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag MBA16_RS14715 Protein ID WP_001307405.1
Coordinates 2960378..2960713 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MBA16_RS14700 (2956163) 2956163..2956933 - 771 WP_001058209.1 2-dehydro-3-deoxyglucarate aldolase -
MBA16_RS14705 (2956949) 2956949..2958283 - 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
MBA16_RS14710 (2958658) 2958658..2960229 + 1572 WP_001273753.1 galactarate dehydratase -
MBA16_RS14715 (2960378) 2960378..2960713 + 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
MBA16_RS14720 (2960713) 2960713..2961177 + 465 WP_000347273.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
MBA16_RS14725 (2961232) 2961232..2962041 - 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
MBA16_RS14730 (2962290) 2962290..2963570 + 1281 WP_000681920.1 tagatose-bisphosphate aldolase subunit KbaZ -
MBA16_RS14735 (2963593) 2963593..2964066 + 474 WP_001336162.1 PTS N-acetylgalactosamine transporter subunit IIB -
MBA16_RS14740 (2964077) 2964077..2964448 + 372 Protein_2874 PTS sugar transporter subunit IIC -
MBA16_RS14745 (2964444) 2964444..2965001 + 558 Protein_2875 amidohydrolase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 2951230..2961177 9947


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17836.25 Da        Isoelectric Point: 9.6924

>T235526 WP_000347273.1 NZ_CP092037:2960713-2961177 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

>T235526 NZ_LR134299:c3759415-3759122 [Pseudomonas putida]
GTGCCACGACTGATCGTCACTGAAGGCGCGGCGAAGGGCCTGGAGCGTTGCCGGCGATTTCTCTCCGATAAAGATCCGCA
GGTCGCACGCCGTGCTGCACAGGCAATCGAGCGCCAATTCGCTCGATTGGAGGAAAGTCCGGAAGTAGGGCGTCCTTTTC
CAGATCTGCCAGAGCTGCGTGAGCTCATCATCGAGTTTGGTGACTCAGGTTACGTCGCGCTGTACCGCTATGAGCGCGCA
GATGACACCGCCTATGTCTTGGCCTTTCGCCATCAAAAAGAAGCTGGCTACTGA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT235526 WP_001307405.1 NZ_CP092037:2960378-2960713 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT235526 NZ_LR134299:c3759675-3759403 [Pseudomonas putida]
ATGGCCACTTCCATCAAAATTGACGACGATCTGAAAGGTCGCATTCAGCATTTGGCTTGTTTGCGCCAGCGCTCATCTCA
CTGGATCATGCGTGAGGCGATCACCCAGTACGTCGAGCGTGAGGAGGCCCGTGAGAGCTTCAAACAAGAAGCGCTGGCAT
CTTGGGCTGCATACCAGGAAACCGGCCAGCACTTGACCGGCCAGGAGGCTCGCACCTGGCTTGGTAGTTGGGGTACAGAT
GCTGAGGCAGAGTTACCCAAGTGCCACGACTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SSH7

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References