105165

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 2414766..2415565 Replicon chromosome
Accession NZ_CP029180
Organism Escherichia coli strain H9Ecoli

Toxin (Protein)


Gene name yhaV Uniprot ID V0SSH7
Locus tag DEF50_RS12965 Protein ID WP_000347273.1
Coordinates 2415101..2415565 (+) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID -
Locus tag DEF50_RS12960 Protein ID WP_072645089.1
Coordinates 2414766..2415101 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DEF50_RS12945 2410551..2411321 - 771 WP_072645090.1 2-dehydro-3-deoxyglucarate aldolase -
DEF50_RS12950 2411337..2412671 - 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
DEF50_RS12955 2413046..2414617 + 1572 WP_001273753.1 galactarate dehydratase -
DEF50_RS12960 2414766..2415101 + 336 WP_072645089.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
DEF50_RS12965 2415101..2415565 + 465 WP_000347273.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
DEF50_RS12970 2415620..2416429 - 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
DEF50_RS12980 2416678..2417958 + 1281 WP_072645088.1 tagatose-bisphosphate aldolase subunit KbaZ -
DEF50_RS12985 2417981..2418454 + 474 WP_001336162.1 PTS N-acetylgalactosamine transporter subunit IIB -
DEF50_RS12990 2418465..2419244 + 780 WP_000406209.1 PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC -
DEF50_RS12995 2419234..2420112 + 879 WP_001295548.1 PTS system mannose/fructose/sorbose family transporter subunit IID -
DEF50_RS13000 2420130..2420564 + 435 WP_000948842.1 PTS sugar transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 2405618..2415565 9947


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17836.25 Da        Isoelectric Point: 9.6924

>T105165 WP_000347273.1 NZ_CP029180:2415101-2415565 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

>T105165 NZ_CP029180:2415101-2415565 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12328.92 Da        Isoelectric Point: 4.8616

>AT105165 WP_072645089.1 NZ_CP029180:2414766-2415101 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKARPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT105165 NZ_CP029180:2414766-2415101 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAGCTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SSH7

Antitoxin

Source ID Structure

References