223792

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 612173..612972 Replicon chromosome
Accession NZ_CP086556
Organism Escherichia coli strain ESY002

Toxin (Protein)


Gene name yhaV Uniprot ID A0A0A6SPA6
Locus tag C5N07_RS02980 Protein ID WP_000347275.1
Coordinates 612173..612637 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag C5N07_RS02985 Protein ID WP_001307405.1
Coordinates 612637..612972 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C5N07_RS02950 (607174) 607174..607608 - 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -
C5N07_RS02955 (607626) 607626..608504 - 879 WP_001295548.1 PTS N-acetylgalactosamine transporter subunit IID -
C5N07_RS02960 (608494) 608494..609273 - 780 WP_000406209.1 PTS N-acetylgalactosamine transporter subunit IIC -
C5N07_RS02965 (609284) 609284..609757 - 474 WP_001336162.1 PTS N-acetylgalactosamine transporter subunit IIB -
C5N07_RS02970 (609780) 609780..611060 - 1281 WP_000681920.1 tagatose-bisphosphate aldolase subunit KbaZ -
C5N07_RS02975 (611309) 611309..612118 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
C5N07_RS02980 (612173) 612173..612637 - 465 WP_000347275.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
C5N07_RS02985 (612637) 612637..612972 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
C5N07_RS02990 (613121) 613121..614692 - 1572 WP_001273752.1 galactarate dehydratase -
C5N07_RS02995 (615067) 615067..616401 + 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
C5N07_RS03000 (616417) 616417..617187 + 771 WP_001058209.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 612173..623847 11674


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17820.29 Da        Isoelectric Point: 9.8492

>T223792 WP_000347275.1 NZ_CP086556:c612637-612173 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKVPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

>T223792 NZ_CP119298:c5364306-5364028 [Pseudomonas aeruginosa]
ATGATTCTGACCTTTCGCTGCGACGAGACTCGTCAGCTTTTTGAGACGGGTCTTTCGAGGCGGTGGGGAGCGATCCTCAC
AGTCGCTACGCGTAAGCTCGCAATGCTTCATGCGGCTAGGGAGCTTCGAGACCTGCGCTCTCCACCTGGAAACCGGTTGG
AGCCGTTGCAGGGAAAGCGGGCGGGCCAACATAGCATCAGGATCAATGACCAGTGGCGTGTCTGTTTCGTCTGGACGGAT
GCGGGTCCCGAAGAAGTCGAAATAGTTGATTACCACTGA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT223792 WP_001307405.1 NZ_CP086556:c612972-612637 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT223792 NZ_CP119298:c5364016-5363711 [Pseudomonas aeruginosa]
ATGGCTACCAATGGTATGCGCCCCATCCATCCTGGGGAAATATTGCGCGATGAGTTTCTGATGGAGTTGGATATCTCTCC
AGCTGCTCTAGCACGCGCTTTGAAAGTCTCCGCTCCGACAGTGAACGATATCGTTCGTGAGCAGCGTGGTATCTCCGCAG
ATATGGCGATTCGTCTGGGGCGTTACTTTGATACGTCCGCTCAGTTCTGGATGAATCTCCAGAGTGAGTATTCGTTAGCA
ACTGCTTATGCGGCGAATGGCAAGCAGATCGAACATGAGATTGAGCCGCTGCTTGCTCACGGATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0A6SPA6

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References