266892

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 79257..80056 Replicon chromosome
Accession NZ_CP114891
Organism Escherichia coli strain CM2

Toxin (Protein)


Gene name yhaV Uniprot ID V0SSH7
Locus tag O6Q44_RS00425 Protein ID WP_000347273.1
Coordinates 79257..79721 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag O6Q44_RS00430 Protein ID WP_001307405.1
Coordinates 79721..80056 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
O6Q44_RS00400 (75433) 75433..75990 - 558 Protein_77 amidohydrolase family protein -
O6Q44_RS00405 (75986) 75986..76357 - 372 Protein_78 PTS sugar transporter subunit IIC -
O6Q44_RS00410 (76368) 76368..76841 - 474 WP_001336162.1 PTS N-acetylgalactosamine transporter subunit IIB -
O6Q44_RS00415 (76864) 76864..78144 - 1281 WP_000681920.1 tagatose-bisphosphate aldolase subunit KbaZ -
O6Q44_RS00420 (78393) 78393..79202 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
O6Q44_RS00425 (79257) 79257..79721 - 465 WP_000347273.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
O6Q44_RS00430 (79721) 79721..80056 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
O6Q44_RS00435 (80205) 80205..81776 - 1572 WP_001273753.1 galactarate dehydratase -
O6Q44_RS00440 (82151) 82151..83485 + 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
O6Q44_RS00445 (83501) 83501..84271 + 771 WP_001058209.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 79257..90931 11674


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17836.25 Da        Isoelectric Point: 9.6924

>T266892 WP_000347273.1 NZ_CP114891:c79721-79257 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT266892 WP_001307405.1 NZ_CP114891:c80056-79721 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SSH7

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References