Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 1870414..1871213 Replicon chromosome
Accession NZ_LR536430
Organism Escherichia coli isolate f974b26a-5e81-11e8-bf7f-3c4a9275d6c8

Toxin (Protein)


Gene name yhaV Uniprot ID D7XZ20
Locus tag E4Z69_RS09545 Protein ID WP_000347264.1
Coordinates 1870749..1871213 (+) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag E4Z69_RS09540 Protein ID WP_001307405.1
Coordinates 1870414..1870749 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E4Z69_RS09525 1866199..1866969 - 771 WP_001058207.1 2-dehydro-3-deoxyglucarate aldolase -
E4Z69_RS09530 1866985..1868319 - 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
E4Z69_RS09535 1868694..1870265 + 1572 WP_001273753.1 galactarate dehydratase -
E4Z69_RS09540 1870414..1870749 + 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
E4Z69_RS09545 1870749..1871213 + 465 WP_000347264.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
E4Z69_RS09550 1871268..1872077 - 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
E4Z69_RS09555 1872326..1873606 + 1281 WP_000681920.1 tagatose-bisphosphate aldolase subunit KbaZ -
E4Z69_RS09560 1873629..1874102 + 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
E4Z69_RS09565 1874113..1874892 + 780 WP_000406214.1 PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC -
E4Z69_RS09570 1874882..1875760 + 879 WP_001300474.1 PTS system mannose/fructose/sorbose family transporter subunit IID -
E4Z69_RS09575 1875778..1876212 + 435 WP_000948824.1 PTS sugar transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 1861371..1871213 9842


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17894.29 Da        Isoelectric Point: 9.4945

>T288392 WP_000347264.1 NZ_LR536430:1870749-1871213 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFDAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT288392 WP_001307405.1 NZ_LR536430:1870414-1870749 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A241QML2

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References