223100

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 4156902..4157701 Replicon chromosome
Accession NZ_CP086342
Organism Escherichia coli strain ESY001

Toxin (Protein)


Gene name yhaV Uniprot ID A0A0A6SPA6
Locus tag C5N06_RS20005 Protein ID WP_000347275.1
Coordinates 4157237..4157701 (+) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag C5N06_RS20000 Protein ID WP_001307405.1
Coordinates 4156902..4157237 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C5N06_RS19985 (4152687) 4152687..4153457 - 771 WP_001058209.1 2-dehydro-3-deoxyglucarate aldolase -
C5N06_RS19990 (4153473) 4153473..4154807 - 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
C5N06_RS19995 (4155182) 4155182..4156753 + 1572 WP_001273752.1 galactarate dehydratase -
C5N06_RS20000 (4156902) 4156902..4157237 + 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
C5N06_RS20005 (4157237) 4157237..4157701 + 465 WP_000347275.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
C5N06_RS20010 (4157756) 4157756..4158565 - 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
C5N06_RS20015 (4158814) 4158814..4160094 + 1281 WP_000681920.1 tagatose-bisphosphate aldolase subunit KbaZ -
C5N06_RS20020 (4160117) 4160117..4160590 + 474 WP_001336162.1 PTS N-acetylgalactosamine transporter subunit IIB -
C5N06_RS20025 (4160601) 4160601..4161380 + 780 WP_000406209.1 PTS N-acetylgalactosamine transporter subunit IIC -
C5N06_RS20030 (4161370) 4161370..4162248 + 879 WP_001295548.1 PTS N-acetylgalactosamine transporter subunit IID -
C5N06_RS20035 (4162266) 4162266..4162700 + 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 4147754..4157701 9947


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17820.29 Da        Isoelectric Point: 9.8492

>T223100 WP_000347275.1 NZ_CP086342:4157237-4157701 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKVPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

>T223100 NZ_CP118494:c1133572-1133264 [Roseovarius tolerans]
GTGACGGAATTTCGGCTGACACGCACAGCGGTGGCCGACCTCGATGATATCTGGGATCATTCAAAGGTAACATGGGGCGA
ACAGCGGGCGGAAAGCTATGTCACGGAGATATTCGCCTGTTTCTCGCGCGCGGCCGCCGCTCCGGAAACAGGGCGCGACC
GGTCCGTCTTCGTGCCGGGTGCGCGGTCACTGCCGGTCGGTCGACATCTTGTCTTCTACCGCATGGTCGGACCGGACGTC
GTGATCTTGCGCGTGGTGCATGAGCGGCGCAACTGGGCGGCCCTCAGTTTCGCATACGATCAGGACTGA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT223100 WP_001307405.1 NZ_CP086342:4156902-4157237 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT223100 NZ_CP118494:c1133826-1133569 [Roseovarius tolerans]
ATGCCCCGCAACACCTCGGTCGTGCTCAGCGACCACTTCAACGACTTCATCGCCCGCGCCGTCGAAAGCGGCCGCTACGC
CTCGACCAGCGACGTCCTGCGCGCGGGGCTGCGGATGCTGGAGCGTCAGGAGGCGGAACTGGAGTTCGACCGCCTGAAAG
CCACCCTTGAGGAGGCCGACCGGCAAGCGGCGAACGGCGAAACCCTGGATGTCGATCTGGAAAGCTTCCTTGCCGCCAAG
CGTAAAACCTCAGCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0A6SPA6

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References