50761

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 3704473..3705272 Replicon chromosome
Accession NZ_CP010143
Organism Escherichia coli strain D4

Toxin (Protein)


Gene name yhaV Uniprot ID A0A0A6SPA6
Locus tag RG37_RS19420 Protein ID WP_000347275.1
Coordinates 3704808..3705272 (+) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag RG37_RS19415 Protein ID WP_001307405.1
Coordinates 3704473..3704808 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RG37_RS19400 3700258..3701028 - 771 WP_001058209.1 2-dehydro-3-deoxyglucarate aldolase -
RG37_RS19405 3701044..3702378 - 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
RG37_RS19410 3702753..3704324 + 1572 WP_001273753.1 galactarate dehydratase -
RG37_RS19415 3704473..3704808 + 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
RG37_RS19420 3704808..3705272 + 465 WP_000347275.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
RG37_RS19425 3705327..3706136 - 810 WP_047600733.1 aga operon transcriptional regulator AgaR -
RG37_RS19435 3706385..3707665 + 1281 WP_000681920.1 tagatose-bisphosphate aldolase subunit KbaZ -
RG37_RS19440 3707688..3708161 + 474 WP_073503457.1 PTS N-acetylgalactosamine transporter subunit IIB -
RG37_RS19445 3708172..3708951 + 780 WP_000406209.1 PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC -
RG37_RS19450 3708941..3709819 + 879 WP_001295548.1 PTS system mannose/fructose/sorbose family transporter subunit IID -
RG37_RS19455 3709837..3710271 + 435 WP_073503458.1 PTS sugar transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 3695326..3705272 9946


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17820.29 Da        Isoelectric Point: 9.8492

>T50761 WP_000347275.1 NZ_CP010143:3704808-3705272 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKVPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

>T50761 NZ_CP010143:3704808-3705272 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGTTCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT50761 WP_001307405.1 NZ_CP010143:3704473-3704808 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT50761 NZ_CP010143:3704473-3704808 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0A6SPA6

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References