Detailed information of TA system TA212487

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 1770017..1770816
• Replicon: chromosome
• Accession: NZ_CP081008
• Organism: Escherichia coli strain XL10-gold-ultracompetent

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: V0SSH7
• Locus tag: K3831_RS08285
• Protein ID: WP_000347273.1
• Coordinates: 1770352..1770816 (+)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: S1EB98
• Locus tag: K3831_RS08280
• Protein ID: WP_001307405.1
• Coordinates: 1770017..1770352 (+)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K3831_RS08265 (1765802)	1765802..1766572	-	771	WP_001058209.1	2-dehydro-3-deoxyglucarate aldolase	-
K3831_RS08270 (1766588)	1766588..1767922	-	1335	WP_000599636.1	galactarate/glucarate/glycerate transporter GarP	-
K3831_RS08275 (1768297)	1768297..1769868	+	1572	WP_001273747.1	galactarate dehydratase	-
K3831_RS08280 (1770017)	1770017..1770352	+	336	WP_001307405.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
K3831_RS08285 (1770352)	1770352..1770816	+	465	WP_000347273.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
K3831_RS08290 (1770871)	1770871..1771680	-	810	WP_000072187.1	aga operon transcriptional regulator AgaR	-
K3831_RS08295 (1771929)	1771929..1773209	+	1281	WP_000681920.1	tagatose-bisphosphate aldolase subunit KbaZ	-
K3831_RS08300 (1773232)	1773232..1773705	+	474	WP_001336162.1	PTS N-acetylgalactosamine transporter subunit IIB	-
K3831_RS08305 (1773716)	1773716..1774087	+	372	Protein_1640	PTS sugar transporter subunit IIC	-
K3831_RS08310 (1774083)	1774083..1774640	+	558	Protein_1641	amidohydrolase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View HG738867 Escherichia coli str. K-12 substr. MC4100 Genomic island TA020080 3504819..3516493 0.004 View NC_000913 Escherichia coli str. K-12 substr. MG1655 Genomic island TA001047 3267854..3277801 0 View NC_007779 Escherichia coli str. K-12 substr. W3110 Genomic island TA021455 3265982..3277656 0.004 View NC_009800 Escherichia coli HS Genomic island TA022436 3314965..3326421 0.008 View NC_010473 Escherichia coli str. K-12 substr. DH10B Genomic island TA022703 3363621..3373568 0 View NC_012759 Escherichia coli BW2952 Genomic island TA023871 3153024..3162971 0 View NC_017638 Escherichia coli DH1 Genomic island TA027421 3245760..3255707 0 View NC_020518 Escherichia coli str. K-12 substr. MDS42 Genomic island TA029016 2741187..2751134 0 View NZ_CP009273 Escherichia coli BW25113 Genomic island TA048700 3261213..3271160 0 View NZ_CP017979 Escherichia coli str. K-12 substr. W3110 strain K-12 substr. ZK126 Genomic island TA068442 3158126..3169800 0.004 View NZ_CP020543 Escherichia coli C Genomic island TA075057 640607..652281 0.005 View NZ_CP025520 Escherichia coli strain DH5alpha Genomic island TA090495 2121277..2131224 0 View NZ_HG738867 Escherichia coli str. K-12 substr. MC4100 strain K-12 Genomic island TA285025 3504819..3516493 0.004	inside	Genomic island	-	-	1760869..1770816	9947

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17836.25 Da
• Isoelectric Point: 9.6924

>T212487 WP_000347273.1 NZ_CP081008:1770352-1770816 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Nucleotide

• Length: 465 bp

>T212487 NZ_CP107064:c6669802-6669215 [Pseudomonas aeruginosa]
ATGTCGAGGAGGGCGCCCATGTTCAAGCCGCTACCGATCACCCTGCAGCGCGGAGCCTTGCGTCTCGAGCCGCTGGTGGA
AGCGGACATTCCCGAGCTGGTGAGCCTCGCCGAAGCCAACCGCGAGGCGCTGCAGTACATGGACGGACCGACGCGGCCGG
ACTGGTACCGCCAGAGCCTCGTCGAGCAGCGCGAGGGCAGGGCGTTGCCGCTCGCCGTCCGCCTCGGCGTGCAACTGGTC
GGGACCACGCGCTTCGCCGAATTCCTTCCCGCCTTGCCGGCCTGCGAGATCGGCTGGACCTGGCTCGACCAGGCCCAGCA
CGGCAGCGGCCTGAACCGCATGATCAAGTACCTGATGCTCAAGCATGCCTTCGACAACCTGCGCATGGTCCGCGTGCAGC
TCAGCACGGCGGCGAGCAACCTGCGCGCCCAGGGCGCCATCGACAAGCTCGGCGCGCAGCGCGAGGGCGTACTGCGCAAC
CACCGGCGGCTGGCCGGCGGGCGGCTAGACGACACCTTCGTCTACAGCATCACCGACCACGAATGGCCGCAGGTCAAGGC
GGCGCTGGAAGCCAGCTTCACCGGCTGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12358.94 Da
• Isoelectric Point: 4.8616

>AT212487 WP_001307405.1 NZ_CP081008:1770017-1770352 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT212487 NZ_CP107064:c6669148-6668294 [Pseudomonas aeruginosa]
GTGCCGGACGAGACGAGCGGAGAACACACGCATTTCTGGCGCGCCGCCGAACTGGGCGGCGTCGAACTGCTGCACGCGCG
CTATGTGGAGCAGCGCTTCGCGCCGCACGTGCACAGCAGCTTCGCCCTGGTCATCATCGAGCAGGGCGCGCAGCGCTTCC
GCCATCGTGGCGGCGAGCACCTGGCCCCGCTGGGCAGCATGGTGCTGATCAACCCCGACGAAGTGCACACCGGCTCCAAG
GCCCATGATGCCGGTTGGCTCTATCGCGGCTACTACCCGGAAATGGAACAGATCGACGGGATTCTCGACGAGCTGGAAAT
CCATCATGGCGGCGTGCCGCGTTTCGCCGACAGCGTGATCCACGACCAGCCCCTGGCACGCGCCTTCGTCCACTTGCACC
GCCTGCTGGCCGAGCATGCCGGCAGCCTGGAAACCCAGACCCGCTGGCGCGAGACGATGCTCGCCCTGTTCCGTCGCCAC
GCCCGCCTGCCAGAAGCGCCGAAGGCCGGGCAGGAGCCGTTGGCGGTGGCCCGCGCCCGCGAACTGCTGGCGGCGCGGTT
GCTCGATCCGCCTTCGCTGGAGGAACTGGCGGCGGCGGTCAATCTGTCCCCGTTCCATTTTGCCCGGGTCTTCCGTCGCG
CCACCGGCATGCCGCCCCACGCCTGGCTCAGACAGCGCCGCCTGGAGCAGGCCAGGGCACTGCTCAAGAGCGGCCGGGCG
CCGCTGGAGGTGGCCCTGGAGCTGGGCTTCGCCGACCAGAGCCACCTCGGTCGCCAGTTCAAGCAGGCCTACGGGGTGGC
ACCGGGTGAATACCGCTCGGCCTGCGCGCGCTCGTCCTTGACGACATACGAATAA

Structures

Toxin

• AlphaFold DB: V0SSH7

Antitoxin

• AlphaFold DB: A0A0E0XVC7