50905

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 3871870..3872669 Replicon chromosome
Accession NZ_CP010152
Organism Escherichia coli strain D9

Toxin (Protein)


Gene name yhaV Uniprot ID A0A0A6SPA6
Locus tag RG42_RS20270 Protein ID WP_000347275.1
Coordinates 3872205..3872669 (+) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag RG42_RS20265 Protein ID WP_001307405.1
Coordinates 3871870..3872205 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RG42_RS20250 3867655..3868425 - 771 WP_001058209.1 2-dehydro-3-deoxyglucarate aldolase -
RG42_RS20255 3868441..3869775 - 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
RG42_RS20260 3870150..3871721 + 1572 WP_001273753.1 galactarate dehydratase -
RG42_RS20265 3871870..3872205 + 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
RG42_RS20270 3872205..3872669 + 465 WP_000347275.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
RG42_RS20275 3872724..3873533 - 810 WP_047600733.1 aga operon transcriptional regulator AgaR -
RG42_RS20285 3873782..3875062 + 1281 WP_000681920.1 tagatose-bisphosphate aldolase subunit KbaZ -
RG42_RS20290 3875085..3875558 + 474 WP_073503457.1 PTS N-acetylgalactosamine transporter subunit IIB -
RG42_RS20295 3875569..3876348 + 780 WP_000406209.1 PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC -
RG42_RS20300 3876338..3877216 + 879 WP_001295548.1 PTS system mannose/fructose/sorbose family transporter subunit IID -
RG42_RS20305 3877234..3877668 + 435 WP_073503458.1 PTS sugar transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 3862723..3872669 9946


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17820.29 Da        Isoelectric Point: 9.8492

>T50905 WP_000347275.1 NZ_CP010152:3872205-3872669 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKVPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

>T50905 NZ_CP010152:3872205-3872669 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGTTCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT50905 WP_001307405.1 NZ_CP010152:3871870-3872205 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT50905 NZ_CP010152:3871870-3872205 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0A6SPA6

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References