282284

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 622115..622914 Replicon chromosome
Accession NZ_CP125731
Organism Escherichia coli strain GYB01

Toxin (Protein)


Gene name yhaV Uniprot ID A0A0A6SPA6
Locus tag QMW19_RS03045 Protein ID WP_000347275.1
Coordinates 622115..622579 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag QMW19_RS03050 Protein ID WP_001307405.1
Coordinates 622579..622914 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QMW19_RS03015 (617116) 617116..617550 - 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -
QMW19_RS03020 (617568) 617568..618446 - 879 WP_001295548.1 PTS N-acetylgalactosamine transporter subunit IID -
QMW19_RS03025 (618436) 618436..619215 - 780 WP_000406209.1 PTS N-acetylgalactosamine transporter subunit IIC -
QMW19_RS03030 (619226) 619226..619699 - 474 WP_001336162.1 PTS N-acetylgalactosamine transporter subunit IIB -
QMW19_RS03035 (619722) 619722..621002 - 1281 WP_000681920.1 tagatose-bisphosphate aldolase subunit KbaZ -
QMW19_RS03040 (621251) 621251..622060 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
QMW19_RS03045 (622115) 622115..622579 - 465 WP_000347275.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
QMW19_RS03050 (622579) 622579..622914 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
QMW19_RS03055 (623063) 623063..624634 - 1572 WP_001273752.1 galactarate dehydratase -
QMW19_RS03060 (625009) 625009..626343 + 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
QMW19_RS03065 (626359) 626359..627129 + 771 WP_001058209.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 622115..633789 11674


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17820.29 Da        Isoelectric Point: 9.8492

>T282284 WP_000347275.1 NZ_CP125731:c622579-622115 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKVPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT282284 WP_001307405.1 NZ_CP125731:c622914-622579 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0A6SPA6

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References