40396

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 607813..608612 Replicon chromosome
Accession NZ_AP025551
Organism Escherichia coli strain LR-50

Toxin (Protein)


Gene name yhaV Uniprot ID V0SSH7
Locus tag QUE19_RS02950 Protein ID WP_000347273.1
Coordinates 607813..608277 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag QUE19_RS02955 Protein ID WP_001307405.1
Coordinates 608277..608612 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QUE19_RS02920 (602814) 602814..603248 - 435 WP_059241785.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -
QUE19_RS02925 (603266) 603266..604144 - 879 WP_001295548.1 PTS N-acetylgalactosamine transporter subunit IID -
QUE19_RS02930 (604134) 604134..604913 - 780 WP_000406209.1 PTS N-acetylgalactosamine transporter subunit IIC -
QUE19_RS02935 (604924) 604924..605397 - 474 WP_001336162.1 PTS N-acetylgalactosamine transporter subunit IIB -
QUE19_RS02940 (605420) 605420..606700 - 1281 WP_000681920.1 tagatose-bisphosphate aldolase subunit KbaZ -
QUE19_RS02945 (606949) 606949..607758 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
QUE19_RS02950 (607813) 607813..608277 - 465 WP_000347273.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
QUE19_RS02955 (608277) 608277..608612 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
QUE19_RS02960 (608761) 608761..610332 - 1572 WP_286330937.1 galactarate dehydratase -
QUE19_RS02965 (610707) 610707..612041 + 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
QUE19_RS02970 (612057) 612057..612827 + 771 WP_001058209.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 607813..619487 11674


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17836.25 Da        Isoelectric Point: 9.6924

>T40396 WP_000347273.1 NZ_AP025551:c608277-607813 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

>T40396 NZ_AP025551:c608277-607813 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT40396 WP_001307405.1 NZ_AP025551:c608612-608277 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT40396 NZ_AP025551:c608612-608277 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SSH7

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References