116331

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 3247023..3247822 Replicon chromosome
Accession NZ_CP034658
Organism Escherichia coli strain ATCC 98082

Toxin (Protein)


Gene name yhaV Uniprot ID V0SSH7
Locus tag EKH78_RS15715 Protein ID WP_000347273.1
Coordinates 3247358..3247822 (+) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag EKH78_RS15710 Protein ID WP_001307405.1
Coordinates 3247023..3247358 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EKH78_RS15695 (3242808) 3242808..3243578 - 771 WP_001058209.1 2-dehydro-3-deoxyglucarate aldolase -
EKH78_RS15700 (3243594) 3243594..3244928 - 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
EKH78_RS15705 (3245303) 3245303..3246874 + 1572 WP_001273753.1 galactarate dehydratase -
EKH78_RS15710 (3247023) 3247023..3247358 + 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
EKH78_RS15715 (3247358) 3247358..3247822 + 465 WP_000347273.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
EKH78_RS15720 (3247877) 3247877..3248686 - 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
EKH78_RS15725 (3248935) 3248935..3250215 + 1281 WP_000681920.1 tagatose-bisphosphate aldolase subunit KbaZ -
EKH78_RS15730 (3250238) 3250238..3250711 + 474 WP_001336162.1 PTS N-acetylgalactosamine transporter subunit IIB -
EKH78_RS15735 (3250722) 3250722..3251093 + 372 Protein_3111 PTS sugar transporter subunit IIC -
EKH78_RS15740 (3251089) 3251089..3251646 + 558 Protein_3112 amidohydrolase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 3237875..3247822 9947


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17836.25 Da        Isoelectric Point: 9.6924

>T116331 WP_000347273.1 NZ_CP034658:3247358-3247822 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

>T116331 NZ_CP034658:3247358-3247822 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT116331 WP_001307405.1 NZ_CP034658:3247023-3247358 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT116331 NZ_CP034658:3247023-3247358 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SSH7

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References