137689

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 4319256..4320055 Replicon chromosome
Accession NZ_CP043199
Organism Escherichia coli O16:H48 strain PG20180060

Toxin (Protein)


Gene name yhaV Uniprot ID V0SSH7
Locus tag FYA11_RS21015 Protein ID WP_000347273.1
Coordinates 4319591..4320055 (+) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag FYA11_RS21010 Protein ID WP_001307405.1
Coordinates 4319256..4319591 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FYA11_RS20995 4315041..4315811 - 771 WP_001058209.1 2-dehydro-3-deoxyglucarate aldolase -
FYA11_RS21000 4315827..4317161 - 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
FYA11_RS21005 4317536..4319107 + 1572 WP_001273753.1 galactarate dehydratase -
FYA11_RS21010 4319256..4319591 + 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
FYA11_RS21015 4319591..4320055 + 465 WP_000347273.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
FYA11_RS21020 4320110..4320919 - 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
FYA11_RS21025 4321168..4322448 + 1281 WP_000681920.1 tagatose-bisphosphate aldolase subunit KbaZ -
FYA11_RS21030 4322471..4322944 + 474 WP_001336162.1 PTS N-acetylgalactosamine transporter subunit IIB -
FYA11_RS21035 4322955..4323326 + 372 Protein_4079 PTS sugar transporter subunit IIC -
FYA11_RS21040 4323322..4323879 + 558 Protein_4080 amidohydrolase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 4310108..4320055 9947


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17836.25 Da        Isoelectric Point: 9.6924

>T137689 WP_000347273.1 NZ_CP043199:4319591-4320055 [Escherichia coli O16:H48]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

>T137689 NZ_CP043199:4319591-4320055 [Escherichia coli O16:H48]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT137689 WP_001307405.1 NZ_CP043199:4319256-4319591 [Escherichia coli O16:H48]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT137689 NZ_CP056899:2996185-2996327 [Citrobacter sp. RHBSTW-00017]
AGATAAAAAGAGACCGAATACAATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTG
GCATTAATGCAGGCTAAGTTACCCTGCCATTTAAGAATAGATGACAGCGCCAGGTTTTCCAGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SSH7

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References