50961

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 1761826..1762625 Replicon chromosome
Accession NZ_CP010160
Organism Escherichia coli strain H1

Toxin (Protein)


Gene name yhaV Uniprot ID V0SSH7
Locus tag RG44_RS09550 Protein ID WP_000347273.1
Coordinates 1762161..1762625 (+) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag RG44_RS09545 Protein ID WP_001307405.1
Coordinates 1761826..1762161 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RG44_RS09530 1757611..1758381 - 771 WP_001058209.1 2-dehydro-3-deoxyglucarate aldolase -
RG44_RS09535 1758397..1759731 - 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
RG44_RS09540 1760106..1761677 + 1572 WP_001273753.1 galactarate dehydratase -
RG44_RS09545 1761826..1762161 + 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
RG44_RS09550 1762161..1762625 + 465 WP_000347273.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
RG44_RS09555 1762680..1763489 - 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
RG44_RS09565 1763738..1765018 + 1281 WP_000681903.1 tagatose-bisphosphate aldolase subunit KbaZ -
RG44_RS09570 1765041..1765514 + 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
RG44_RS09575 1765525..1766304 + 780 WP_000406214.1 PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC -
RG44_RS09580 1766294..1767172 + 879 WP_073544745.1 PTS system mannose/fructose/sorbose family transporter subunit IID -
RG44_RS09585 1767190..1767624 + 435 WP_000948824.1 PTS sugar transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 1752678..1762625 9947


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17836.25 Da        Isoelectric Point: 9.6924

>T50961 WP_000347273.1 NZ_CP010160:1762161-1762625 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

>T50961 NZ_CP010160:1762161-1762625 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT50961 WP_001307405.1 NZ_CP010160:1761826-1762161 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT50961 NZ_CP010160:1761826-1762161 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SSH7

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References