186054

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 3768810..3769609 Replicon chromosome
Accession NZ_CP067303
Organism Escherichia coli strain ABW_A32

Toxin (Protein)


Gene name yhaV Uniprot ID A0A0A6SPA6
Locus tag JJB23_RS18020 Protein ID WP_000347275.1
Coordinates 3769145..3769609 (+) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag JJB23_RS18015 Protein ID WP_001307405.1
Coordinates 3768810..3769145 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JJB23_RS18000 (3764595) 3764595..3765365 - 771 WP_001058209.1 2-dehydro-3-deoxyglucarate aldolase -
JJB23_RS18005 (3765381) 3765381..3766715 - 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
JJB23_RS18010 (3767090) 3767090..3768661 + 1572 WP_001273752.1 galactarate dehydratase -
JJB23_RS18015 (3768810) 3768810..3769145 + 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
JJB23_RS18020 (3769145) 3769145..3769609 + 465 WP_000347275.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
JJB23_RS18025 (3769664) 3769664..3770473 - 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
JJB23_RS18030 (3770722) 3770722..3772002 + 1281 WP_000681920.1 tagatose-bisphosphate aldolase subunit KbaZ -
JJB23_RS18035 (3772025) 3772025..3772498 + 474 WP_001336162.1 PTS N-acetylgalactosamine transporter subunit IIB -
JJB23_RS18040 (3772509) 3772509..3773288 + 780 WP_000406209.1 PTS N-acetylgalactosamine transporter subunit IIC -
JJB23_RS18045 (3773278) 3773278..3774156 + 879 WP_001295548.1 PTS N-acetylgalactosamine transporter subunit IID -
JJB23_RS18050 (3774174) 3774174..3774608 + 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 3759662..3769609 9947


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17820.29 Da        Isoelectric Point: 9.8492

>T186054 WP_000347275.1 NZ_CP067303:3769145-3769609 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKVPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

>T186054 NZ_CP089162:32053-32157 [Staphylococcus aureus]
TTGTTACTCCTAGAAAGGACTAGCATGTCTGATTTTGAAATGCTTATGGTTGTATTAACAATCATTGGTTTAGTATTGAT
TAGTACTCAAGACCATAAAAAATAA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT186054 WP_001307405.1 NZ_CP067303:3768810-3769145 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT186054 NZ_CP089162:c32316-32152 [Staphylococcus aureus]
GTAGTAAGTAGAAGCAAAAGATGAAAATCATTAACTCTTGAAACACAAAAAGGGCAACACTCGGAAACATGTTACCCTAA
TGAGCCCGTTAAAAAGACGGTGACCTTATATTTTATTTAAAAATAGCCTTCAAAAATGCCGGTCAAAGCGAATAGAAGGT
TATTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0A6SPA6

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References