60762

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 2055424..2056223 Replicon chromosome
Accession NZ_CP014348
Organism Escherichia coli str. K-12 substr. MG1655 strain JW5437-1

Toxin (Protein)


Gene name yhaV Uniprot ID V0SSH7
Locus tag AWN69_RS10165 Protein ID WP_000347273.1
Coordinates 2055424..2055888 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag AWN69_RS10170 Protein ID WP_001307405.1
Coordinates 2055888..2056223 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AWN69_RS10140 2051600..2052157 - 558 Protein_1920 amidohydrolase family protein -
AWN69_RS10145 2052153..2052524 - 372 Protein_1921 PTS sugar transporter subunit IIC -
AWN69_RS10150 2052535..2053008 - 474 WP_001336162.1 PTS N-acetylgalactosamine transporter subunit IIB -
AWN69_RS10155 2053031..2054311 - 1281 WP_000681920.1 tagatose-bisphosphate aldolase subunit KbaZ -
AWN69_RS10160 2054560..2055369 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
AWN69_RS10165 2055424..2055888 - 465 WP_000347273.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
AWN69_RS10170 2055888..2056223 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
AWN69_RS10175 2056372..2057943 - 1572 WP_001273753.1 galactarate dehydratase -
AWN69_RS10180 2058318..2059652 + 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
AWN69_RS10185 2059668..2060438 + 771 WP_001058209.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 2055424..2067098 11674


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17836.25 Da        Isoelectric Point: 9.6924

>T60762 WP_000347273.1 NZ_CP014348:c2055888-2055424 [Escherichia coli str. K-12 substr. MG1655]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

>T60762 NZ_CP014348:c2055888-2055424 [Escherichia coli str. K-12 substr. MG1655]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT60762 WP_001307405.1 NZ_CP014348:c2056223-2055888 [Escherichia coli str. K-12 substr. MG1655]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT60762 NZ_CP014348:c2056223-2055888 [Escherichia coli str. K-12 substr. MG1655]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SSH7

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References