197272

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 3504819..3505618 Replicon chromosome
Accession NZ_CP072054
Organism Escherichia coli strain K1508

Toxin (Protein)


Gene name yhaV Uniprot ID V0SSH7
Locus tag J5N95_RS16890 Protein ID WP_000347273.1
Coordinates 3504819..3505283 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag J5N95_RS16895 Protein ID WP_001307405.1
Coordinates 3505283..3505618 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J5N95_RS16865 (3500995) 3500995..3501552 - 558 Protein_3329 amidohydrolase family protein -
J5N95_RS16870 (3501548) 3501548..3501919 - 372 Protein_3330 PTS sugar transporter subunit IIC -
J5N95_RS16875 (3501930) 3501930..3502403 - 474 WP_001336162.1 PTS N-acetylgalactosamine transporter subunit IIB -
J5N95_RS16880 (3502426) 3502426..3503706 - 1281 WP_000681920.1 tagatose-bisphosphate aldolase subunit KbaZ -
J5N95_RS16885 (3503955) 3503955..3504764 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
J5N95_RS16890 (3504819) 3504819..3505283 - 465 WP_000347273.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
J5N95_RS16895 (3505283) 3505283..3505618 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
J5N95_RS16900 (3505767) 3505767..3507338 - 1572 WP_001273753.1 galactarate dehydratase -
J5N95_RS16905 (3507713) 3507713..3509047 + 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
J5N95_RS16910 (3509063) 3509063..3509833 + 771 WP_001058209.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 3504819..3516493 11674


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17836.25 Da        Isoelectric Point: 9.6924

>T197272 WP_000347273.1 NZ_CP072054:c3505283-3504819 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

>T197272 NZ_CP095856:4097326-4097724 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACAGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT197272 WP_001307405.1 NZ_CP072054:c3505618-3505283 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT197272 NZ_CP095856:4097030-4097323 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATTACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SSH7

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References