157194

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 3218548..3219347 Replicon chromosome
Accession NZ_CP053607
Organism Escherichia coli strain NEB5-alpha_F'Iq

Toxin (Protein)


Gene name yhaV Uniprot ID V0SSH7
Locus tag HO392_RS15630 Protein ID WP_000347273.1
Coordinates 3218883..3219347 (+) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag HO392_RS15625 Protein ID WP_001307405.1
Coordinates 3218548..3218883 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HO392_RS15610 3214333..3215103 - 771 WP_001058209.1 2-dehydro-3-deoxyglucarate aldolase -
HO392_RS15615 3215119..3216453 - 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
HO392_RS15620 3216828..3218399 + 1572 WP_001273747.1 galactarate dehydratase -
HO392_RS15625 3218548..3218883 + 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
HO392_RS15630 3218883..3219347 + 465 WP_000347273.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
HO392_RS15635 3219402..3220211 - 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
HO392_RS15640 3220460..3221740 + 1281 WP_000681920.1 tagatose-bisphosphate aldolase subunit KbaZ -
HO392_RS15645 3221763..3222236 + 474 WP_001336162.1 PTS N-acetylgalactosamine transporter subunit IIB -
HO392_RS15650 3222247..3222618 + 372 Protein_3055 PTS sugar transporter subunit IIC -
HO392_RS15655 3222614..3223171 + 558 Protein_3056 amidohydrolase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 3209400..3219347 9947


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17836.25 Da        Isoelectric Point: 9.6924

>T157194 WP_000347273.1 NZ_CP053607:3218883-3219347 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

>T157194 NZ_CP069078:671672-672079 [Mycobacterium tuberculosis]
GTGATCATCGACACGAGTGCGCTGCTTGCCTATTTCGACGCCGCCGAGCCAGACCACGCCGCAGTGTCTGAGTGCATCGA
TAGCTCCGCAGACGCGCTCGTCGTATCCCCTTATGTGGTAGCGGAACTCGACTATCTCGTCGCCACCCGGGTAGGTGTCG
ATGCCGAGCTCGCCGTCCTGCGTGAACTCGCCGGCGGGGCCTGGGAGCTCGCCAACTGCGGTGCCGCCGAAATCGAGCAG
GCCGCCCGCATCGTCACGAAATACCAGGATCAGCGGATCGGGATCGCGGATGCGGCCAACGTCGTGCTGGCCGACCGATA
CCGCACGCGCACGATCCTCACCCTGGACCGTCGGCACTTCTCGGCGCTGCGGCCGATCGGCGGTGGGCGCTTCACCGTCA
TTCCGTAA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT157194 WP_001307405.1 NZ_CP053607:3218548-3218883 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT157194 NZ_CP069078:671460-671675 [Mycobacterium tuberculosis]
GTGGACAAGACGACGGTCTACCTGCCGGATGAACTCAAGGCGGCCGTGAAGCGCGCCGCTCGGCAGCGCGGAGTCTCCGA
AGCGCAGGTAATCCGGGAGTCCATCCGGGCGGCGGTCGGCGGCGCCAAGCCGCCGCCGCGCGGGGGTCTATATGCGGGTT
CGGAGCCCATCGCGCGGCGAGTCGACGAGCTGCTGGCTGGCTTCGGTGAGCGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SSH7

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References