204309

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 607036..607835 Replicon chromosome
Accession NZ_CP075722
Organism Escherichia coli strain CPE35

Toxin (Protein)


Gene name yhaV Uniprot ID A0A0A6SPA6
Locus tag KKZ10_RS02960 Protein ID WP_000347275.1
Coordinates 607036..607500 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag KKZ10_RS02965 Protein ID WP_001307405.1
Coordinates 607500..607835 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KKZ10_RS02930 (602037) 602037..602471 - 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -
KKZ10_RS02935 (602489) 602489..603367 - 879 WP_001295548.1 PTS N-acetylgalactosamine transporter subunit IID -
KKZ10_RS02940 (603357) 603357..604136 - 780 WP_000406209.1 PTS N-acetylgalactosamine transporter subunit IIC -
KKZ10_RS02945 (604147) 604147..604620 - 474 WP_001336162.1 PTS N-acetylgalactosamine transporter subunit IIB -
KKZ10_RS02950 (604643) 604643..605923 - 1281 WP_000681920.1 tagatose-bisphosphate aldolase subunit KbaZ -
KKZ10_RS02955 (606172) 606172..606981 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
KKZ10_RS02960 (607036) 607036..607500 - 465 WP_000347275.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
KKZ10_RS02965 (607500) 607500..607835 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
KKZ10_RS02970 (607984) 607984..609555 - 1572 WP_001273752.1 galactarate dehydratase -
KKZ10_RS02975 (609930) 609930..611264 + 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
KKZ10_RS02980 (611280) 611280..612050 + 771 WP_001058209.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 607036..618710 11674


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17820.29 Da        Isoelectric Point: 9.8492

>T204309 WP_000347275.1 NZ_CP075722:c607500-607036 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKVPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

>T204309 NZ_CP100695:c4297103-4296819 [Salmonella enterica subsp. enterica serovar Weltevreden]
ATGACTTATGAACTGGAATTCGACCCGAGGGCCTTAAAAGAGTGGCATAAGCTGGGCGATACGGTGAAGGCTCAGCTTAA
GAAAAAGTTGGCTGATGTGCTATTGAACCCCAGAATCGACTCTGCCCGTTTAAACGGTCTTCCTGACTGCTATAAAATTA
AGCTTAAATCGTCCGGTTATCGCCTGGTGTACCAGGTTCGGGATGAAGTTGTGATTGTGTTTGTTGTTGCGGTCGGCAAG
CGAGAACATTCAGCCGTCTATCACGATGCAAACAAACGGCTTTAG

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT204309 WP_001307405.1 NZ_CP075722:c607835-607500 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT204309 NZ_CP100695:c4297335-4297093 [Salmonella enterica subsp. enterica serovar Weltevreden]
ATGGCCACGCTGAACGTCCGTCTGGATGACAAACTCAAAAATGAGGCTTATGCCGTGCTGGAAAAACTGAACATTACTCC
AACGGAAGCCGTGCGATTACTGTTCCAGTACGTCGCGGAAACGGGGCGCATGCCAGTGAAAACCGTCACTCTTAGCGACA
GTGAAGATGCATTGATTCAGACAGTTCGGGAGAGGCTGTCTAGTCCGCAAAAGGGAATCAAGGTTAGTCTGGATGACTTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0A6SPA6

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References