103504

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 4106287..4107086 Replicon chromosome
Accession NZ_CP028748
Organism Escherichia coli strain A27

Toxin (Protein)


Gene name yhaV Uniprot ID V0SSH7
Locus tag AOY80_RS19750 Protein ID WP_000347273.1
Coordinates 4106622..4107086 (+) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag AOY80_RS19745 Protein ID WP_001307405.1
Coordinates 4106287..4106622 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AOY80_RS19730 (4102072) 4102072..4102842 - 771 WP_001058209.1 2-dehydro-3-deoxyglucarate aldolase -
AOY80_RS19735 (4102858) 4102858..4104192 - 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
AOY80_RS19740 (4104567) 4104567..4106138 + 1572 WP_001273753.1 galactarate dehydratase -
AOY80_RS19745 (4106287) 4106287..4106622 + 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
AOY80_RS19750 (4106622) 4106622..4107086 + 465 WP_000347273.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
AOY80_RS19755 (4107141) 4107141..4107950 - 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
AOY80_RS19760 (4108199) 4108199..4109479 + 1281 WP_000681920.1 tagatose-bisphosphate aldolase subunit KbaZ -
AOY80_RS19765 (4109502) 4109502..4109975 + 474 WP_001336162.1 PTS N-acetylgalactosamine transporter subunit IIB -
AOY80_RS19770 (4109986) 4109986..4110765 + 780 WP_000406209.1 PTS N-acetylgalactosamine transporter subunit IIC -
AOY80_RS19775 (4110755) 4110755..4111633 + 879 WP_001295548.1 PTS N-acetylgalactosamine transporter subunit IID -
AOY80_RS19780 (4111651) 4111651..4112085 + 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 4096230..4107086 10856


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17836.25 Da        Isoelectric Point: 9.6924

>T103504 WP_000347273.1 NZ_CP028748:4106622-4107086 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

>T103504 NZ_CP028748:4106622-4107086 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT103504 WP_001307405.1 NZ_CP028748:4106287-4106622 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT103504 NZ_CP028748:4106287-4106622 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SSH7

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References