213152

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 632834..633633 Replicon chromosome
Accession NZ_CP081711
Organism Escherichia coli strain AH21

Toxin (Protein)


Gene name yhaV Uniprot ID V0SSH7
Locus tag J6O42_RS03105 Protein ID WP_000347273.1
Coordinates 632834..633298 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag J6O42_RS03110 Protein ID WP_001307405.1
Coordinates 633298..633633 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J6O42_RS03075 (627835) 627835..628269 - 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -
J6O42_RS03080 (628287) 628287..629165 - 879 WP_001295548.1 PTS N-acetylgalactosamine transporter subunit IID -
J6O42_RS03085 (629155) 629155..629934 - 780 WP_000406209.1 PTS N-acetylgalactosamine transporter subunit IIC -
J6O42_RS03090 (629945) 629945..630418 - 474 WP_001336162.1 PTS N-acetylgalactosamine transporter subunit IIB -
J6O42_RS03095 (630441) 630441..631721 - 1281 WP_000681920.1 tagatose-bisphosphate aldolase subunit KbaZ -
J6O42_RS03100 (631970) 631970..632779 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
J6O42_RS03105 (632834) 632834..633298 - 465 WP_000347273.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
J6O42_RS03110 (633298) 633298..633633 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
J6O42_RS03115 (633782) 633782..635353 - 1572 WP_001273753.1 galactarate dehydratase -
J6O42_RS03120 (635728) 635728..637062 + 1335 WP_061357358.1 galactarate/glucarate/glycerate transporter GarP -
J6O42_RS03125 (637078) 637078..637848 + 771 WP_001058209.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 632834..644507 11673


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17836.25 Da        Isoelectric Point: 9.6924

>T213152 WP_000347273.1 NZ_CP081711:c633298-632834 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

>T213152 NZ_CP107473:3059772-3060140 [Klebsiella pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCAGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACTCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCGGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCGGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGCTTGCGCGGATGA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT213152 WP_001307405.1 NZ_CP081711:c633633-633298 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT213152 NZ_CP107473:3059554-3059775 [Klebsiella pneumoniae]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGACGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SSH7

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References