255261

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 603634..604433 Replicon chromosome
Accession NZ_CP103515
Organism Escherichia coli strain 5444

Toxin (Protein)


Gene name yhaV Uniprot ID A0A0A6SPA6
Locus tag M5S96_RS02950 Protein ID WP_000347275.1
Coordinates 603634..604098 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag M5S96_RS02955 Protein ID WP_001307405.1
Coordinates 604098..604433 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M5S96_RS02920 (598635) 598635..599069 - 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -
M5S96_RS02925 (599087) 599087..599965 - 879 WP_001295548.1 PTS N-acetylgalactosamine transporter subunit IID -
M5S96_RS02930 (599955) 599955..600734 - 780 WP_000406209.1 PTS N-acetylgalactosamine transporter subunit IIC -
M5S96_RS02935 (600745) 600745..601218 - 474 WP_001336162.1 PTS N-acetylgalactosamine transporter subunit IIB -
M5S96_RS02940 (601241) 601241..602521 - 1281 WP_000681920.1 tagatose-bisphosphate aldolase subunit KbaZ -
M5S96_RS02945 (602770) 602770..603579 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
M5S96_RS02950 (603634) 603634..604098 - 465 WP_000347275.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
M5S96_RS02955 (604098) 604098..604433 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
M5S96_RS02960 (604582) 604582..606153 - 1572 WP_001273752.1 galactarate dehydratase -
M5S96_RS02965 (606528) 606528..607862 + 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
M5S96_RS02970 (607878) 607878..608648 + 771 WP_001058209.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 603634..615308 11674


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17820.29 Da        Isoelectric Point: 9.8492

>T255261 WP_000347275.1 NZ_CP103515:c604098-603634 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKVPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT255261 WP_001307405.1 NZ_CP103515:c604433-604098 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0A6SPA6

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References